3KJ7

Crystal Structure of the Complex of C-lobe of Bovine Lactoferrin with Dextrin at 1.9 A Resolution

Summary for 3KJ7

• Entry DOI: 10.2210/pdb3kj7/pdb
• Related: 2DWA 2DXY 3IBO
• Related PRD ID: PRD_900014
• Descriptor: Lactotransferrin, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (9 entities in total)
• Functional Keywords: complex, dextrin, c-lobe, antibiotic, antimicrobial, disulfide bond, glycoprotein, hydrolase, ion transport, iron, iron transport, metal-binding, phosphoprotein, protease, secreted, serine protease, transport
• Biological source: Bos taurus (bovine,cow,domestic cattle,domestic cow)
• Total number of polymer chains: 1
• Total formula weight: 40100.16

Authors

Mir, R.,Vikram, G.,Singh, N.,Sinha, M.,Sharma, S.,Kaur, P.,Singh, T.P. (deposition date: 2009-11-03, release date: 2010-02-16, Last modification date: 2023-09-06)

Primary citation

Mir, R.,Kumar, R.P.,Singh, N.,Vikram, G.P.,Sinha, M.,Bhushan, A.,Kaur, P.,Srinivasan, A.,Sharma, S.,Singh, T.P.
Specific interactions of C-terminal half (C-lobe) of lactoferrin protein with edible sugars: binding and structural studies with implications on diabetes.
Int.J.Biol.Macromol., 47:50-59, 2010

PubMed Abstract: Bovine lactoferrin has been shown to reduce the levels of glucose in both normal subjects and non-insulin dependent diabetic patients. The binding studies have shown that various sugar molecules interact with lactoferrin indicating the presence of a sugar-binding site in the protein. Structural studies have revealed that the sugar-binding site is located in the C-terminal half (C-lobe) of bilobal lactoferrin. Since the sugar-binding site was part of the C-lobe, it was better to carry out binding and structural studies using C-lobe rather than the full protein molecule. Therefore, C-lobe was prepared by limited proteolysis of lactoferrin with enzyme proteinase K. It was purified to homogeneity for further studies. The addition of C-lobe to human serum showed significant lowering of glucose levels. The binding studies using C-lobe with nine sugars, glucose, galactose, mannose, xylose, maltose, cellobiose, lactose, sucrose and dextrin gave values of binding constants in the range of 10(-4) to 10(-5)M. The structure determinations of the complexes of C-lobe with all the nine sugars showed that all of them interact with C-lobe through the same recognition site involving several hydrogen bonds and van der Waals interactions.

PubMed: 20371371
DOI: 10.1016/j.ijbiomac.2010.03.021

Experimental method

X-RAY DIFFRACTION (1.91 Å)