3KIJ

Crystal structure of the human PDI-peroxidase

Summary for 3KIJ

• Entry DOI: 10.2210/pdb3kij/pdb
• Descriptor: Probable glutathione peroxidase 8, SULFATE ION (3 entities in total)
• Functional Keywords: human pdi-peroxidase, glutathione peroxidase, membrane, oxidoreductase, peroxidase, transmembrane
• Biological source: Homo sapiens (human)
• Cellular location: Membrane ; Single-pass membrane protein : Q8TED1
• Total number of polymer chains: 3
• Total formula weight: 63190.11

Authors

Nguyen, D.V.,Ruddock, L.W. (deposition date: 2009-11-02, release date: 2011-01-12, Last modification date: 2023-09-06)

Primary citation

Nguyen, V.D.,Saaranen, M.J.,Karala, A.R.,Lappi, A.K.,Wang, L.,Raykhel, I.B.,Alanen, H.I.,Salo, K.E.,Wang, C.C.,Ruddock, L.W.
Two Endoplasmic Reticulum PDI Peroxidases Increase the Efficiency of the Use of Peroxide during Disulfide Bond Formation.
J.Mol.Biol., 406:503-515, 2011

PubMed Abstract: Disulfide bond formation in the endoplasmic reticulum by the sulfhydryl oxidase Ero1 family is thought to be accompanied by the concomitant formation of hydrogen peroxide. Since secretory cells can make substantial amounts of proteins that contain disulfide bonds, the production of this reactive oxygen species could have potentially lethal consequences. Here, we show that two human proteins, GPx7 and GPx8, labeled as secreted glutathione peroxidases, are actually endoplasmic reticulum-resident protein disulfide isomerase peroxidases. In vitro, the addition of GPx7 or GPx8 to a folding protein along with protein disulfide isomerase and peroxide enables the efficient oxidative refolding of a reduced denatured protein. Furthermore, both GPx7 and GPx8 interact with Ero1α in vivo, and GPx7 significantly increases oxygen consumption by Ero1α in vitro. Hence, GPx7 and GPx8 may represent a novel route for the productive use of peroxide produced by Ero1α during disulfide bond formation.

PubMed: 21215271
DOI: 10.1016/j.jmb.2010.12.039

Experimental method

X-RAY DIFFRACTION (1.8 Å)