3KDM
Crystal Structure of Human Anti-steroid Fab 5F2 in Complex with Testosterone
Summary for 3KDM
| Entry DOI | 10.2210/pdb3kdm/pdb |
| Descriptor | Immunoglobulin light chain, Immunoglobulin heavy chain, TESTOSTERONE, ... (4 entities in total) |
| Functional Keywords | immune system, antibody, immunoglobulin fab fragment, anti-steroid |
| Biological source | Homo sapiens More |
| Total number of polymer chains | 4 |
| Total formula weight | 94777.22 |
| Authors | Niemi, M.H.,Rouvinen, J. (deposition date: 2009-10-23, release date: 2010-05-26, Last modification date: 2024-11-20) |
| Primary citation | Niemi, M.H.,Takkinen, K.,Amundsen, L.K.,Soderlund, H.,Rouvinen, J.,Hoyhtya, M. The testosterone binding mechanism of an antibody derived from a naive human scFv library J.Mol.Recognit., 24:209-219, 2010 Cited by PubMed Abstract: A testosterone binding scFv antibody was isolated from a naïve human library with a modest size of 10(8) clones. The crystal structure of the Fab fragment form of the 5F2 antibody clone complexed with testosterone determined at 1.5 Å resolution shows that the hapten is bound deeply in the antibody binding pocket. In addition to the interactions with framework residues only CDR-L3 and CDR-H3 loops interact with testosterone and the heavy chain forms the majority of the contacts with the hapten. The testosterone binding site of the 5F2 antibody with a high abundance of aromatic amino acid residues shows similarity with an in vitro affinity matured antibody having around 300 times higher affinity. The moderate affinity of the 5F2 antibody originates from the different orientation of the hapten and few light chain contacts. This is the first three-dimensional structure of a human steroid hormone binding antibody that has been isolated from a naïve human repertoire. PubMed: 21360611DOI: 10.1002/jmr.1039 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.5 Å) |
Structure validation
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