3KDE

Crystal structure of the THAP domain from D. melanogaster P-element transposase in complex with its natural DNA binding site

3KDE の概要

• エントリーDOI: 10.2210/pdb3kde/pdb
• 分子名称: 5'-D(*GP*TP*TP*AP*AP*GP*(BRU)P*GP*GP*A)-3', 5'-D(*(BRU)P*CP*CP*AP*CP*TP*TP*AP*AP*C)-3', Transposable element P transposase, ... (5 entities in total)
• 機能のキーワード: thap domain, dna-binding domain, zinc-finger, beta-alpha-beta, p-element transposase, dna integration, dna recombination, dna-binding, metal-binding, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Drosophila melanogaster (Fruit fly); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 14969.44

構造登録者

Sabogal, A.,Lyubimov, A.Y.,Berger, J.M.,Rio, D.C. (登録日: 2009-10-22, 公開日: 2009-12-08, 最終更新日: 2024-02-21)

主引用文献

Sabogal, A.,Lyubimov, A.Y.,Corn, J.E.,Berger, J.M.,Rio, D.C.
THAP proteins target specific DNA sites through bipartite recognition of adjacent major and minor grooves.
Nat.Struct.Mol.Biol., 17:117-123, 2010

PubMed Abstract: THAP-family C(2)CH zinc-coordinating DNA-binding proteins function in diverse eukaryotic cellular processes, such as transposition, transcriptional repression, stem-cell pluripotency, angiogenesis and neurological function. To determine the molecular basis for sequence-specific DNA recognition by THAP proteins, we solved the crystal structure of the Drosophila melanogaster P element transposase THAP domain (DmTHAP) in complex with a natural 10-base-pair site. In contrast to C(2)H(2) zinc fingers, DmTHAP docks a conserved beta-sheet into the major groove and a basic C-terminal loop into the adjacent minor groove. We confirmed specific protein-DNA interactions by mutagenesis and DNA-binding assays. Sequence analysis of natural and in vitro-selected binding sites suggests that several THAPs (DmTHAP and human THAP1 and THAP9) recognize a bipartite TXXGGGX(A/T) consensus motif; homology suggests THAP proteins bind DNA through a bipartite interaction. These findings reveal the conserved mechanisms by which THAP-family proteins engage specific chromosomal target elements.

PubMed: 20010837
DOI: 10.1038/nsmb.1742

実験手法

X-RAY DIFFRACTION (1.74 Å)