3KCH

Baranase crosslinked by glutaraldehyde

Summary for 3KCH

• Entry DOI: 10.2210/pdb3kch/pdb
• Related: 1A2P
• Descriptor: Ribonuclease, PENTANEDIAL (3 entities in total)
• Functional Keywords: ribonuclease, microbial ribonuclease, glutaraldehyde, crosslink, endonuclease, hydrolase, nuclease, secreted
• Biological source: Bacillus amyloliquefaciens
• Cellular location: Secreted: P00648
• Total number of polymer chains: 3
• Total formula weight: 37496.51

Authors

Prange, T.,Salem, M.,Mauguen, Y. (deposition date: 2009-10-21, release date: 2010-03-09, Last modification date: 2024-11-20)

Primary citation

Salem, M.,Mauguen, Y.,Prange, T.
Revisiting glutaraldehyde cross-linking: the case of the Arg-Lys intermolecular doublet
Acta Crystallogr.,Sect.F, 66:225-228, 2010

PubMed Abstract: In addition to the common use of glutaraldehyde to nonspecifically cross-link protein crystals through lysine residues disposed on the surface of the protein, the use of gentle vapour diffusion of glutaraldehyde offers a convenient way to limit polymerization and to allow slow diffusion throughout the crystal. In the case of trimeric barnase crystals, a specific cross-link was observed between an lysine side chain and an arginine side chain that were spatially disposed at the ideal distance on the protein surface in the three monomers. Here, the direct observation of a specific Lys-Arg cross-link site is reported and a mechanism is proposed for the reaction.

PubMed: 20208148
DOI: 10.1107/S1744309109054037

Experimental method

X-RAY DIFFRACTION (1.94 Å)