3K91

Polysulfane Bridge in Cu-Zn Superoxide Dismutase

3K91 の概要

• エントリーDOI: 10.2210/pdb3k91/pdb
• 関連するPDBエントリー: 2NNX 3GQF
• 分子名称: Superoxide dismutase [Cu-Zn], PENTASULFIDE-SULFUR (3 entities in total)
• 機能のキーワード: human cu-zn superoxide dismutase, amyotrophic lateral sclerosis, antioxidant, oxidoreductase, sulfur, polysulfane, disease mutation, disulfide bond, metal-binding, neurodegeneration, phosphoprotein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P00441
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 31833.51

構造登録者

You, Z.,Cao, X.,Taylor, A.B.,Hart, P.J.,Levine, R.L. (登録日: 2009-10-15, 公開日: 2010-01-19, 最終更新日: 2024-11-27)

主引用文献

You, Z.,Cao, X.,Taylor, A.B.,Hart, P.J.,Levine, R.L.
Characterization of a covalent polysulfane bridge in copper-zinc superoxide dismutase .
Biochemistry, 49:1191-1198, 2010

PubMed Abstract: In the course of studies on human copper-zinc superoxide dismutase (SOD1), we observed a modified form of the protein whose mass was increased by 158 mass units. The covalent modification was characterized, and we established that it is a novel heptasulfane bridge connecting the two Cys111 residues in the SOD1 homodimer. The heptasulfane bridge was visualized directly in the crystal structure of a recombinant human mutant SOD1, H46R/H48Q, produced in yeast. The modification is reversible, with the bridge being cleaved by thiols, by cyanide, and by unfolding of the protein to expose the polysulfane. The polysulfane bridge can be introduced in vitro by incubation of purified SOD1 with elemental sulfur, even under anaerobic conditions and in the presence of a metal chelator. Because polysulfanes and polysulfides can catalyze the generation of reactive oxygen and sulfur species, the modification may endow SOD1 with a toxic gain of function.

PubMed: 20052996
DOI: 10.1021/bi901844d

実験手法

X-RAY DIFFRACTION (1.75 Å)