3K70

Crystal structure of the complete initiation complex of RecBCD

3K70 の概要

• エントリーDOI: 10.2210/pdb3k70/pdb
• 関連するPDBエントリー: 1W36
• 分子名称: Exodeoxyribonuclease V beta chain, Exodeoxyribonuclease V gamma chain, Exodeoxyribonuclease V alpha chain, ... (5 entities in total)
• 機能のキーワード: recombination, helicase, nuclease, hydrolase, dna repair, atp-binding, dna damage, endonuclease, exonuclease, nucleotide-binding, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Escherichia coli K-12; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 693488.09

構造登録者

Saikrishnan, K.,Wigley, D.B. (登録日: 2009-10-11, 公開日: 2009-11-10, 最終更新日: 2023-09-06)

主引用文献

Saikrishnan, K.,Griffiths, S.P.,Cook, N.,Court, R.,Wigley, D.B.
DNA binding to RecD: role of the 1B domain in SF1B helicase activity.
Embo J., 27:2222-2229, 2008

PubMed Abstract: The molecular mechanism of superfamily 1Balpha helicases remains unclear. We present here the crystal structure of the RecD2 helicase from Deinococcus radiodurans at 2.2-A resolution. The structure reveals the folds of the 1B and 2B domains of RecD that were poorly ordered in the structure of the Escherichia coli RecBCD enzyme complex reported previously. The 2B domain adopts an SH3 fold which, although common in eukaryotes, is extremely rare in bacterial systems. In addition, the D. radiodurans RecD2 structure has aided us in deciphering lower resolution (3.6 A) electron density maps for the E. coli RecBCD enzyme in complex with a long DNA substrate that interacts with the RecD subunit. Taken together, these structures indicated an important role for the 1B domain of RecD, a beta-hairpin that extends from the surface of the 1A domain and interacts with the DNA substrate. On the basis of these structural data, we designed a mutant RecD2 helicase that lacks this pin. The 'pin-less' mutant protein is a fully active ssDNA-dependent ATPase but totally lacks helicase activity.

PubMed: 18668125

実験手法

X-RAY DIFFRACTION (3.59 Å)