3JXT

Crystal structure of the third PDZ domain of SAP-102 in complex with a fluorogenic peptide-based ligand

3JXT の概要

• エントリーDOI: 10.2210/pdb3jxt/pdb
• 分子名称: Disks large homolog 3, Voltage-dependent calcium channel gamma-2 subunit, ACETATE ION, ... (4 entities in total)
• 機能のキーワード: sap102, dlg3, stargazin, 4-dmap, 4db, pdz domain, solvatochromic flurophore, fluorogenic probe, calcium channel, calcium transport, ion transport, ionic channel, transport, voltage-gated channel, signaling protein
• 由来する生物種: Rattus norvegicus (rat); 詳細
• 細胞内の位置: Membrane; Multi-pass membrane protein: O88602
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 24095.75

構造登録者

Sainlos, M.,Olivier, N.B.,Imperiali, B. (登録日: 2009-09-21, 公開日: 2010-09-29, 最終更新日: 2024-11-20)

主引用文献

Sainlos, M.,Iskenderian-Epps, W.S.,Olivier, N.B.,Choquet, D.,Imperiali, B.
Caged mono- and divalent ligands for light-assisted disruption of PDZ domain-mediated interactions.
J.Am.Chem.Soc., 135:4580-4583, 2013

PubMed Abstract: We report a general method for light-assisted control of interactions of PDZ domain binding motifs with their cognate domains by the incorporation of a photolabile caging group onto the essential C-terminal carboxylate binding determinant of the motif. The strategy was implemented and validated for both simple monovalent and biomimetic divalent ligands, which have recently been established as powerful tools for acute perturbation of native PDZ domain-dependent interactions in live cells.

PubMed: 23480637
DOI: 10.1021/ja309870q

実験手法

X-RAY DIFFRACTION (1.5 Å)