3JU0

Structure of the arm-type binding domain of HAI7 integrase

Summary for 3JU0

• Entry DOI: 10.2210/pdb3ju0/pdb
• Related: 3JTZ
• Descriptor: Phage integrase (2 entities in total)
• Functional Keywords: four stranded beta-sheet, dna binding protein
• Biological source: Pectobacterium atrosepticum (Pectobacterium atrosepticum)
• Total number of polymer chains: 1
• Total formula weight: 12528.37

Authors

Szwagierczak, A.,Antonenka, U.,Popowicz, G.M.,Sitar, T.,Holak, T.A.,Rakin, A. (deposition date: 2009-09-14, release date: 2009-10-06, Last modification date: 2023-11-01)

Primary citation

Szwagierczak, A.,Antonenka, U.,Popowicz, G.M.,Sitar, T.,Holak, T.A.,Rakin, A.
Structures of the arm-type binding domains of HPI and HAI7 integrases
J.Biol.Chem., 284:31664-31671, 2009

PubMed Abstract: The structures of the N-terminal domains of two integrases of closely related but not identical asn tDNA-associated genomic islands, Yersinia HPI (high pathogenicity island; encoding siderophore yersiniabactin biosynthesis and transport) and an Erwinia carotovora genomic island with yet unknown function, HAI7, have been resolved. Both integrases utilize a novel four-stranded beta-sheet DNA-binding motif, in contrast to the known proteins that bind their DNA targets by means of three-stranded beta-sheets. Moreover, the beta-sheets in Int(HPI) and Int(HAI7) are longer than those in other integrases, and the structured helical N terminus is positioned perpendicularly to the large C-terminal helix. These differences strongly support the proposal that the integrases of the genomic islands make up a distinct evolutionary branch of the site-specific recombinases that utilize a unique DNA-binding mechanism.

PubMed: 19737930
DOI: 10.1074/jbc.M109.059261

Experimental method

X-RAY DIFFRACTION (1.6 Å)