3JTZ
Structure of the arm-type binding domain of HPI integrase
Summary for 3JTZ
| Entry DOI | 10.2210/pdb3jtz/pdb |
| Related | 3JU0 |
| Descriptor | Integrase, SODIUM ION (3 entities in total) |
| Functional Keywords | four stranded beta-sheet, dna binding protein |
| Biological source | Yersinia pestis |
| Total number of polymer chains | 1 |
| Total formula weight | 10125.69 |
| Authors | Szwagierczak, A.,Antonenka, U.,Popowicz, G.M.,Sitar, T.,Holak, T.A.,Rakin, A. (deposition date: 2009-09-14, release date: 2009-10-06, Last modification date: 2024-03-20) |
| Primary citation | Szwagierczak, A.,Antonenka, U.,Popowicz, G.M.,Sitar, T.,Holak, T.A.,Rakin, A. Structures of the arm-type binding domains of HPI and HAI7 integrases J.Biol.Chem., 284:31664-31671, 2009 Cited by PubMed Abstract: The structures of the N-terminal domains of two integrases of closely related but not identical asn tDNA-associated genomic islands, Yersinia HPI (high pathogenicity island; encoding siderophore yersiniabactin biosynthesis and transport) and an Erwinia carotovora genomic island with yet unknown function, HAI7, have been resolved. Both integrases utilize a novel four-stranded beta-sheet DNA-binding motif, in contrast to the known proteins that bind their DNA targets by means of three-stranded beta-sheets. Moreover, the beta-sheets in Int(HPI) and Int(HAI7) are longer than those in other integrases, and the structured helical N terminus is positioned perpendicularly to the large C-terminal helix. These differences strongly support the proposal that the integrases of the genomic islands make up a distinct evolutionary branch of the site-specific recombinases that utilize a unique DNA-binding mechanism. PubMed: 19737930DOI: 10.1074/jbc.M109.059261 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.3 Å) |
Structure validation
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