3JTZ
Structure of the arm-type binding domain of HPI integrase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-26 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9873 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 48.750, 48.750, 74.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.300 |
| R-factor | 0.2138 |
| Rwork | 0.212 |
| R-free | 0.23867 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.280 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.400 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.036 | 0.103 |
| Number of reflections | 19463 | |
| <I/σ(I)> | 29.07 | 8.98 |
| Completeness [%] | 85.0 | 65.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 0.1M tri-sodium citrate, pH5.6, 35% tert-butanol, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
