3JTZ

Structure of the arm-type binding domain of HPI integrase

3JTZ の概要

• エントリーDOI: 10.2210/pdb3jtz/pdb
• 関連するPDBエントリー: 3JU0
• 分子名称: Integrase, SODIUM ION (3 entities in total)
• 機能のキーワード: four stranded beta-sheet, dna binding protein
• 由来する生物種: Yersinia pestis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10125.69

構造登録者

Szwagierczak, A.,Antonenka, U.,Popowicz, G.M.,Sitar, T.,Holak, T.A.,Rakin, A. (登録日: 2009-09-14, 公開日: 2009-10-06, 最終更新日: 2024-03-20)

主引用文献

Szwagierczak, A.,Antonenka, U.,Popowicz, G.M.,Sitar, T.,Holak, T.A.,Rakin, A.
Structures of the arm-type binding domains of HPI and HAI7 integrases
J.Biol.Chem., 284:31664-31671, 2009

PubMed Abstract: The structures of the N-terminal domains of two integrases of closely related but not identical asn tDNA-associated genomic islands, Yersinia HPI (high pathogenicity island; encoding siderophore yersiniabactin biosynthesis and transport) and an Erwinia carotovora genomic island with yet unknown function, HAI7, have been resolved. Both integrases utilize a novel four-stranded beta-sheet DNA-binding motif, in contrast to the known proteins that bind their DNA targets by means of three-stranded beta-sheets. Moreover, the beta-sheets in Int(HPI) and Int(HAI7) are longer than those in other integrases, and the structured helical N terminus is positioned perpendicularly to the large C-terminal helix. These differences strongly support the proposal that the integrases of the genomic islands make up a distinct evolutionary branch of the site-specific recombinases that utilize a unique DNA-binding mechanism.

PubMed: 19737930
DOI: 10.1074/jbc.M109.059261

実験手法

X-RAY DIFFRACTION (1.3 Å)