3JTH

Crystal structure of a transcriptional regulator HlyU from Vibrio vulnificus CMCP6

3JTH の概要

• エントリーDOI: 10.2210/pdb3jth/pdb
• 分子名称: Transcription activator HlyU (2 entities in total)
• 機能のキーワード: transcription factor, hlyu, rtxa, dna-binding, transcription, transcription regulation
• 由来する生物種: Vibrio vulnificus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 22903.60

構造登録者

Nishi, K.,Lee, H.J.,Park, S.Y.,Kim, J.S. (登録日: 2009-09-12, 公開日: 2010-03-16, 最終更新日: 2024-11-13)

主引用文献

Nishi, K.,Lee, H.J.,Park, S.Y.,Bae, S.J.,Lee, S.E.,Adams, P.D.,Rhee, J.H.,Kim, J.S.
Crystal structure of the transcriptional activator HlyU from Vibrio vulnificus CMCP6.
Febs Lett., 2010

PubMed Abstract: HlyU is a transcription factor of the ArsR/SmtB family and activates the expression of the pathogenic Vibrio vulnificus RTX toxin. In contrast to the other metal-responding ArsR/SmtB proteins, HlyU does not sense metal ions. To provide its structural information, we elucidated the crystal structure of HlyU from V. vulnificus CMCP6 (HlyU_Vv). The monomeric HlyU_Vv architecture of five alpha-helices and two beta-strands, some of which constitute a typical DNA-binding winged helix-turn-helix (wHTH) motif, is very similar to that of other transcription regulators. Nonetheless, the homo-dimeric HlyU_Vv structure shows several different, three-dimensional features in the spatial position and the detailed dimeric interaction, which were not observed in the modeling study based on the same protein family and sequence similarity.

PubMed: 20178784
DOI: 10.1016/j.febslet.2010.02.052

実験手法

X-RAY DIFFRACTION (2 Å)