3JSM

K65R mutant HIV-1 reverse transcriptase cross-linked to DS-DNA and complexed with tenofovir-diphosphate as the incoming nucleotide substrate

3JSM の概要

• エントリーDOI: 10.2210/pdb3jsm/pdb
• 関連するPDBエントリー: 1RTD 1T05 3JYT
• 分子名称: DNA (5'-D(*A*TP*GP*GP*TP*CP*GP*GP*CP*GP*CP*CP*CP*GP*AP*AP*CP*AP*GP*GP*GP*AP*CP*TP*GP*TP*G)-3'), DNA (5'-D(*A*CP*AP*GP*TP*CP*CP*CP*TP*GP*TP*TP*CP*GP*GP*(MRG)P*CP*GP*CP*CP*(DDG))-3'), HIV-1 REVERSE TRANSCRIPTASE P66 SUBUNIT, ... (8 entities in total)
• 機能のキーワード: hiv-1 reverse transcriptase, tenofovir, rt-dna complex, transferase-dna complex, drug resistance mutation, aids, dna recombination, dna-directed dna polymerase, rnase h, hydrolase, lipoprotein, magnesium, membrane, metal-binding, multifunctional enzyme, nucleotidyltransferase, rna-directed dna polymerase transferase, transferase-dna complex complex, transferase/dna complex
• 由来する生物種: Human immunodeficiency virus type 1 (HIV-1); 詳細
• 細胞内の位置: Matrix protein p17: Virion (Potential). Capsid protein p24: Virion (Potential). Nucleocapsid protein p7: Virion (Potential). Reverse transcriptase/ribonuclease H: Virion (Potential). Integrase: Virion (Potential): P03366 P03366
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 130918.85

構造登録者

Das, K.,Arnold, E. (登録日: 2009-09-10, 公開日: 2009-09-29, 最終更新日: 2023-09-06)

• 主引用文献: Das, K.,Bandwar, R.P.,White, K.L.,Feng, J.Y.,Sarafianos, S.G.,Tuske, S.,Tu, X.,Clark, A.D.,Boyer, P.L.,Hou, X.,Gaffney, B.L.,Jones, R.A.,Miller, M.D.,Hughes, S.H.,Arnold, E.; Structural basis for the role of the K65r mutation in HIV-1 reverse transcriptase polymerization, excision antagonism, and tenofovir resistance.; J.Biol.Chem., 284:35092-35100, 2009; PubMed: 19812032; DOI: 10.1074/jbc.M109.022525

実験手法

X-RAY DIFFRACTION (3 Å)