3JSB

Crystal structure of the N-terminal domain of the Lymphocytic Choriomeningitis Virus L protein

3JSB の概要

• エントリーDOI: 10.2210/pdb3jsb/pdb
• 分子名称: RNA-directed RNA polymerase (2 entities in total)
• 機能のキーワード: viral protein, vizier, structural genomics, marseilles structural genomics program @ afmb, msgp, nucleotide-binding, nucleotidyltransferase, rna replication, rna-directed rna polymerase, transferase, virion, rna binding protein
• 由来する生物種: Lymphocytic choriomeningitis virus (LCMV)
• 細胞内の位置: Virion: P14240
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47444.34

構造登録者

Morin, B.,Jamal, S.,Ferron, F.P.,Coutard, B.,Bricogne, G.,Canard, B.,Vonrhein, C.,Marseilles Structural Genomics Program @ AFMB (MSGP) (登録日: 2009-09-10, 公開日: 2010-09-15, 最終更新日: 2024-03-20)

主引用文献

Morin, B.,Coutard, B.,Lelke, M.,Ferron, F.P.,Kerber, R.,Jamal, S.,Frangeul, A.,Baronti, C.,Charrel, R.,de Lamballerie, X.,Vonrhein, C.,Lescar, J.,Bricogne, G.,Gunther, S.,Canard, B.
The N-terminal domain of the arenavirus L protein is an RNA endonuclease essential in mRNA transcription
Plos Pathog., 6:e1001038-e1001038, 2010

PubMed Abstract: Arenaviridae synthesize viral mRNAs using short capped primers presumably acquired from cellular transcripts by a 'cap-snatching' mechanism. Here, we report the crystal structure and functional characterization of the N-terminal 196 residues (NL1) of the L protein from the prototypic arenavirus: lymphocytic choriomeningitis virus. The NL1 domain is able to bind and cleave RNA. The 2.13 Å resolution crystal structure of NL1 reveals a type II endonuclease α/β architecture similar to the N-terminal end of the influenza virus PA protein. Superimposition of both structures, mutagenesis and reverse genetics studies reveal a unique spatial arrangement of key active site residues related to the PD…(D/E)XK type II endonuclease signature sequence. We show that this endonuclease domain is conserved and active across the virus families Arenaviridae, Bunyaviridae and Orthomyxoviridae and propose that the arenavirus NL1 domain is the Arenaviridae cap-snatching endonuclease.

PubMed: 20862324
DOI: 10.1371/journal.ppat.1001038

実験手法

X-RAY DIFFRACTION (2.13 Å)