3JC2

The structure of the mammalian Sec61 channel opened by a signal sequence

3JC2 の概要

• エントリーDOI: 10.2210/pdb3jc2/pdb
• EMDBエントリー: 3245
• 分子名称: Protein transport protein Sec61 subunit alpha isoform 1, Protein transport protein Sec61 subunit gamma, Prolactin, ... (4 entities in total)
• 機能のキーワード: sec61, translocation, signal sequence, transport protein
• 由来する生物種: Bos taurus (bovine); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 64093.78

構造登録者

Voorhees, R.M.,Hegde, R.S. (登録日: 2015-11-15, 公開日: 2016-01-13, 最終更新日: 2025-03-26)

主引用文献

Voorhees, R.M.,Hegde, R.S.
Structure of the Sec61 channel opened by a signal sequence.
Science, 351:88-91, 2016

PubMed Abstract: Secreted and integral membrane proteins compose up to one-third of the biological proteome. These proteins contain hydrophobic signals that direct their translocation across or insertion into the lipid bilayer by the Sec61 protein-conducting channel. The molecular basis of how hydrophobic signals within a nascent polypeptide trigger channel opening is not understood. Here, we used cryo-electron microscopy to determine the structure of an active Sec61 channel that has been opened by a signal sequence. The signal supplants helix 2 of Sec61α, which triggers a rotation that opens the central pore both axially across the membrane and laterally toward the lipid bilayer. Comparisons with structures of Sec61 in other states suggest a pathway for how hydrophobic signals engage the channel to gain access to the lipid bilayer.

PubMed: 26721998
DOI: 10.1126/science.aad4992

実験手法

ELECTRON MICROSCOPY (3.6 Å)