3JA5

Genome and RdRp structure within the capsid of no-transcribing cypovirus

Summary for 3JA5

• Entry DOI: 10.2210/pdb3ja5/pdb
• Related: 3JA4
• EMDB information: 6322
• Descriptor: RNA-dependent RNA polymerase (1 entity in total)
• Functional Keywords: non-transcribing cypovirus, rna-dependent rna polymerase, dsrna virus, icosahedral virus, transferase
• Biological source: Bombyx mori cypovirus 1 (BmCPV)
• Total number of polymer chains: 1
• Total formula weight: 138830.11

Authors

Liu, H.,Cheng, L. (deposition date: 2015-04-21, release date: 2015-10-28, Last modification date: 2024-03-20)

Primary citation

Liu, H.,Cheng, L.
Cryo-EM shows the polymerase structures and a nonspooled genome within a dsRNA virus.
Science, 349:1347-1350, 2015

PubMed Abstract: Double-stranded RNA (dsRNA) viruses possess a segmented dsRNA genome and a number of RNA-dependent RNA polymerases (RdRps) enclosed in a capsid. Until now, the precise structures of genomes and RdRps within the capsids have been unknown. Here we report the structures of RdRps and associated RNAs within nontranscribing and transcribing cypoviruses (NCPV and TCPV, respectively), using a combination of cryo-electron microscopy (cryo-EM) and a symmetry-mismatch reconstruction method. The RdRps and associated RNAs appear to exhibit a pseudo-D3 symmetric organization in both NCPV and TCPV. However, the molecular interactions between RdRps and the genomic RNA were found to differ in these states. Our work provides insight into the mechanisms of the replication and transcription in dsRNA viruses and paves a way for structural determination of lower-symmetry complexes enclosed in higher-symmetry structures.

PubMed: 26383954
DOI: 10.1126/science.aaa4938

Experimental method

ELECTRON MICROSCOPY (12 Å)