3J9R

Atomic structures of a bactericidal contractile nanotube in its pre- and post-contraction states

This is a large structure.

> Summary

Summary for 3J9R

Related3J9Q
EMDB informationEMD-6271 EMD-6270
Descriptorsheath
Functional Keywordspyocin, bacteriocin, sheath, tube, structural protein
Biological sourcePseudomonas aeruginosa
Total number of polymer chains36
Total molecular weight1484919.58
Authors
Ge, P.,Scholl, D.,Leiman, P.G.,Yu, X.,Miller, J.F.,Zhou, Z.H. (deposition date: 2015-02-17, release date: 2015-04-01, Last modification date: 2015-05-20)
Primary citation
Ge, P.,Scholl, D.,Leiman, P.G.,Yu, X.,Miller, J.F.,Zhou, Z.H.
Atomic structures of a bactericidal contractile nanotube in its pre- and postcontraction states.
Nat.Struct.Mol.Biol., 22:377-382, 2015
PubMed: 25822993
DOI: 10.1038/nsmb.2995
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY
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Structure validation

ClashscoreRamachandran outliersSidechain outliers34.7%1.4%MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures

More Asymmetric unit images

Molmil generated image of 3j9r
no rotation
Molmil generated image of 3j9r
rotated about x axis by 90°
Molmil generated image of 3j9r
rotated about y axis by 90°

> Structural details

Entity

Chain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
A, B, F, E, D...sheathpolymer38641247.836
UniProt (Q9S574)
Pfam (PF04984)
Pfam (PF17482)
Pseudomonas aeruginosaSimilar to FI genes of P2, phiCTX, and PS17: tail sheath

Sequence viewer

Contents of the asymmetric unit

PolymersNumber of chains36
Total molecular weight1484919.6
Non-Polymers*Number of molecules0
Total molecular weight0.0
All*Total molecular weight1484919.6
*Water molecules are not included.

> Experimental details

Refinement Statistics

Experimental method:ELECTRON MICROSCOPY

Cell axes1.0001.0001.000
Cell angles90.0090.0090.00
SpacegroupP 1
Resolution limits -
the highest resolution shell value -

Data Collection Statistics

Resolution limits -
the highest resolution shell value -

Crystallization Conditions

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Crystallization Reagents

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Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
Annotated Information is extracted from Literature Info*

> Functional details

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Functional Information from GO Data

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Functional Information from PDB Data

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Functional Information from PDB atom coordinates for the "HETATM" binding sites

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Functional Information from PROSITE/UniProt

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Functional Information from SwissProt/UniProt

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Catalytic Information from CSA

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> Sequence Neighbor

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