3J9G

Atomic model of the VipA/VipB, the type six secretion system contractile sheath of Vibrio cholerae from cryo-EM

This is a large structure.

> Summary

Summary for 3J9G

EMDB informationEMD-2699
DescriptorVipA, VipB
Functional Keywordst6ss, bacterial secretion, phage, contraction, contractile protein
Biological sourceVibrio cholerae O1 biovar El Tor str. N16961
Total number of polymer chains60
Total molecular weight1886541.99
Authors
Primary citation
Kudryashev, M.,Wang, R.Y.,Brackmann, M.,Scherer, S.,Maier, T.,Baker, D.,DiMaio, F.,Stahlberg, H.,Egelman, E.H.,Basler, M.
Structure of the Type VI Secretion System Contractile Sheath.
Cell(Cambridge,Mass.), 160:952-962, 2015
PubMed: 25723169
DOI: 10.1016/j.cell.2015.01.037
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY
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Structure validation

ClashscoreRamachandran outliersSidechain outliers10.7%0MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures

More Asymmetric unit images

Molmil generated image of 3j9g
no rotation
Molmil generated image of 3j9g
rotated about x axis by 90°
Molmil generated image of 3j9g
rotated about y axis by 90°

> Structural details

Sequence viewer

Contents of the asymmetric unit

PolymersNumber of chains60
Total molecular weight1886542.0
Non-Polymers*Number of molecules0
Total molecular weight0.0
All*Total molecular weight1886542.0
*Water molecules are not included.

> Experimental details

Refinement Statistics

Experimental method:ELECTRON MICROSCOPY

Cell axes1.0001.0001.000
Cell angles90.0090.0090.00
SpacegroupP 1
Resolution limits -
the highest resolution shell value -

Data Collection Statistics

Resolution limits -
the highest resolution shell value -

Crystallization Conditions

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Crystallization Reagents

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Crystallization Reagents in Literatures*
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Annotated Information is extracted from Literature Info*

> Functional details

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Functional Information from GO Data

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Functional Information from PDB Data

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Functional Information from PDB atom coordinates for the "HETATM" binding sites

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Functional Information from PROSITE/UniProt

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Functional Information from SwissProt/UniProt

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Catalytic Information from CSA

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> Sequence Neighbor

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