3J46

Structure of the SecY protein translocation channel in action

3J46 の概要

• エントリーDOI: 10.2210/pdb3j46/pdb
• 関連するPDBエントリー: 2I2P 3I8G 3J01 3J45
• EMDBエントリー: 5692 5693
• 分子名称: Protein translocase subunit SecY, 50S ribosomal protein L29P, 23S ribosomal RNA, ... (14 entities in total)
• 機能のキーワード: 70s, preprotein translocase, secyeg, protein translocation channel, nascent chain, ribosome-protein transport complex, ribosome/protein transport
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 256080.53

構造登録者

Akey, C.W.,Park, E.,Menetret, J.F.,Gumbart, J.C.,Ludtke, S.J.,Li, W.,Whynot, A.,Rapoport, T.A. (登録日: 2013-06-18, 公開日: 2013-10-23, 最終更新日: 2024-11-27)

主引用文献

Park, E.,Menetret, J.F.,Gumbart, J.C.,Ludtke, S.J.,Li, W.,Whynot, A.,Rapoport, T.A.,Akey, C.W.
Structure of the SecY channel during initiation of protein translocation.
Nature, 506:102-106, 2013

PubMed Abstract: Many secretory proteins are targeted by signal sequences to a protein-conducting channel, formed by prokaryotic SecY or eukaryotic Sec61 complexes, and are translocated across the membrane during their synthesis. Crystal structures of the inactive channel show that the SecY subunit of the heterotrimeric complex consists of two halves that form an hourglass-shaped pore with a constriction in the middle of the membrane and a lateral gate that faces the lipid phase. The closed channel has an empty cytoplasmic funnel and an extracellular funnel that is filled with a small helical domain, called the plug. During initiation of translocation, a ribosome-nascent chain complex binds to the SecY (or Sec61) complex, resulting in insertion of the nascent chain. However, the mechanism of channel opening during translocation is unclear. Here we have addressed this question by determining structures of inactive and active ribosome-channel complexes with cryo-electron microscopy. Non-translating ribosome-SecY channel complexes derived from Methanocaldococcus jannaschii or Escherichia coli show the channel in its closed state, and indicate that ribosome binding per se causes only minor changes. The structure of an active E. coli ribosome-channel complex demonstrates that the nascent chain opens the channel, causing mostly rigid body movements of the amino- and carboxy-terminal halves of SecY. In this early translocation intermediate, the polypeptide inserts as a loop into the SecY channel with the hydrophobic signal sequence intercalated into the open lateral gate. The nascent chain also forms a loop on the cytoplasmic surface of SecY rather than entering the channel directly.

PubMed: 24153188
DOI: 10.1038/nature12720

実験手法

ELECTRON MICROSCOPY (10.1 Å)