3J35

Cryo-EM reconstruction of Dengue virus at 37 C

Summary for 3J35

• Entry DOI: 10.2210/pdb3j35/pdb
• EMDB information: 5587
• Descriptor: envelope protein (1 entity in total)
• Functional Keywords: bumpy structure, conformational change, temperature dependent, virus
• Biological source: Dengue virus 2
• Total number of polymer chains: 3
• Total formula weight: 131370.92

Authors

Zhang, X.Z.,Sheng, J.,Plevka, P.,Kuhn, R.J.,Diamond, M.S.,Rossmann, M.G. (deposition date: 2013-02-24, release date: 2013-04-10, Last modification date: 2024-02-21)

Primary citation

Zhang, X.,Sheng, J.,Plevka, P.,Kuhn, R.J.,Diamond, M.S.,Rossmann, M.G.
Dengue structure differs at the temperatures of its human and mosquito hosts.
Proc.Natl.Acad.Sci.USA, 110:6795-6799, 2013

PubMed Abstract: We report on a conformational transition of dengue virus when changing the temperature from that present in its mosquito vectors to that of its human host. Using cryoelectron microscopy, we show that although the virus has a smooth surface, a diameter of ∼500 Å, and little exposed membrane at room temperature, the virions have a bumpy appearance with a diameter of ∼550 Å and some exposed membrane at 37 °C. The bumpy structure at 37 °C was found to be similar to the previously predicted structure of an intermediate between the smooth mature and fusogenic forms. As humans have a body temperature of 37 °C, the bumpy form of the virus would be the form present in humans. Thus, optimal dengue virus vaccines should induce antibodies that preferentially recognize epitopes exposed on the bumpy form of the virus.

PubMed: 23569243
DOI: 10.1073/pnas.1304300110

Experimental method

ELECTRON MICROSCOPY (35 Å)