3J0P

Core of mammalian 80S pre-ribosome in complex with tRNAs fitted to a 10.6A cryo-em map: rotated PRE state 1

3J0P の概要

• エントリーDOI: 10.2210/pdb3j0p/pdb
• 関連するPDBエントリー: 3J0L 3J0O 3J0Q
• EMDBエントリー: 5328
• 分子名称: 40S ribosomal RNA fragment, Ribosomal protein S30, 60S ribosomal RNA fragment, ... (18 entities in total)
• 機能のキーワード: mammalia, translation, elongation cycle, trna, ribosome
• 由来する生物種: Oryctolagus cuniculus (rabbit); 詳細
• タンパク質・核酸の鎖数: 18
• 化学式量合計: 247719.64

構造登録者

Budkevich, T.,Giesebrecht, J.,Altman, R.,Munro, J.,Mielke, T.,Nierhaus, K.,Blanchard, S.,Spahn, C.M. (登録日: 2011-10-06, 公開日: 2011-11-16, 最終更新日: 2024-02-21)

主引用文献

Budkevich, T.,Giesebrecht, J.,Altman, R.B.,Munro, J.B.,Mielke, T.,Nierhaus, K.H.,Blanchard, S.C.,Spahn, C.M.
Structure and dynamics of the Mammalian ribosomal pretranslocation complex.
Mol.Cell, 44:214-224, 2011

PubMed Abstract: Although the structural core of the ribosome is conserved in all kingdoms of life, eukaryotic ribosomes are significantly larger and more complex than their bacterial counterparts. The extent to which these differences influence the molecular mechanism of translation remains elusive. Multiparticle cryo-electron microscopy and single-molecule FRET investigations of the mammalian pretranslocation complex reveal spontaneous, large-scale conformational changes, including an intersubunit rotation of the ribosomal subunits. Through structurally related processes, tRNA substrates oscillate between classical and at least two distinct hybrid configurations facilitated by localized changes in their L-shaped fold. Hybrid states are favored within the mammalian complex. However, classical tRNA positions can be restored by tRNA binding to the E site or by the eukaryotic-specific antibiotic and translocation inhibitor cycloheximide. These findings reveal critical distinctions in the structural and energetic features of bacterial and mammalian ribosomes, providing a mechanistic basis for divergent translation regulation strategies and species-specific antibiotic action.

PubMed: 22017870
DOI: 10.1016/j.molcel.2011.07.040

実験手法

ELECTRON MICROSCOPY (10.6 Å)