3IZ3
CryoEM structure of cytoplasmic polyhedrosis virus
Summary for 3IZ3
| Entry DOI | 10.2210/pdb3iz3/pdb |
| EMDB information | 5233 |
| Descriptor | Structural protein VP3, Structural protein VP1, Viral structural protein 5 (3 entities in total) |
| Functional Keywords | cryoelectron microscopy, cytoplasmic polyhedrosis virus, reovirus, transcription, icosahedral virus, virus |
| Biological source | Bombyx mori cypovirus 1 (BmCPV) More |
| Total number of polymer chains | 5 |
| Total formula weight | 482187.46 |
| Authors | |
| Primary citation | Cheng, L.,Sun, J.,Zhang, K.,Mou, Z.,Huang, X.,Ji, G.,Sun, F.,Zhang, J.,Zhu, P. Atomic model of a cypovirus built from cryo-EM structure provides insight into the mechanism of mRNA capping. Proc.Natl.Acad.Sci.USA, 108:1373-1378, 2011 Cited by PubMed Abstract: The cytoplasmic polyhedrosis virus (CPV) from the family Reoviridae belongs to a subgroup of "turreted" reoviruses, in which the mRNA capping activity occurs in a pentameric turret. We report a full atomic model of CPV built from a 3D density map obtained using cryoelectron microscopy. The image data for the 3D reconstruction were acquired exclusively from a CCD camera. Our structure shows that the enzymatic domains of the pentameric turret of CPV are topologically conserved and that there are five unique channels connecting the guanylyltransferase and methyltransferase regions. This structural organization reveals how the channels guide nascent mRNA sequentially to guanylyltransferase, 7-N-methyltransferase, and 2'-O-methyltransferase in the turret, undergoing the highly coordinated mRNA capping activity. Furthermore, by fitting the deduced amino acid sequence of the protein VP5 to 120 large protrusion proteins on the CPV capsid shell, we confirmed that this protrusion protein is encoded by CPV RNA segment 7. PubMed: 21220303DOI: 10.1073/pnas.1014995108 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.9 Å) |
Structure validation
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