3IY3

Variable domains of the computer generated model (WAM) of Fab 8 fitted into the cryoEM reconstruction of the virus-Fab 8 complex

3IY3 の概要

• エントリーDOI: 10.2210/pdb3iy3/pdb
• EMDBエントリー: 5108
• 分子名称: antibody fragment from neutralizing antibody 8 (light chain), antibody fragment from neutralizing antibody 8 (heavy chain) (2 entities in total)
• 機能のキーワード: cryoem, neutralizing antibody, parvovirus, canine, feline, fab footprint, immune system
• 由来する生物種: Mus musculus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 25381.36

構造登録者

Hafenstein, S.,Bowman, V.D.,Sun, T.,Nelson, C.D.,Palermo, L.M.,Chipman, P.R.,Battisti, A.J.,Parrish, C.R.,Rossmann, M.G. (登録日: 2009-04-09, 公開日: 2009-05-12, 最終更新日: 2024-10-09)

主引用文献

Hafenstein, S.,Bowman, V.D.,Sun, T.,Nelson, C.D.,Palermo, L.M.,Chipman, P.R.,Battisti, A.J.,Parrish, C.R.,Rossmann, M.G.
Structural comparison of different antibodies interacting with parvovirus capsids
J.Virol., 83:5556-5566, 2009

PubMed Abstract: The structures of canine parvovirus (CPV) and feline parvovirus (FPV) complexed with antibody fragments from eight different neutralizing monoclonal antibodies were determined by cryo-electron microscopy (cryoEM) reconstruction to resolutions varying from 8.5 to 18 A. The crystal structure of one of the Fab molecules and the sequence of the variable domain for each of the Fab molecules have been determined. The structures of Fab fragments not determined crystallographically were predicted by homology modeling according to the amino acid sequence. Fitting of the Fab and virus structures into the cryoEM densities identified the footprints of each antibody on the viral surface. As anticipated from earlier analyses, the Fab binding sites are directed to two epitopes, A and B. The A site is on an exposed part of the surface near an icosahedral threefold axis, whereas the B site is about equidistant from the surrounding five-, three-, and twofold axes. One antibody directed to the A site binds CPV but not FPV. Two of the antibodies directed to the B site neutralize the virus as Fab fragments. The differences in antibody properties have been linked to the amino acids within the antibody footprints, the position of the binding site relative to the icosahedral symmetry elements, and the orientation of the Fab structure relative to the surface of the virus. Most of the exposed surface area was antigenic, although each of the antibodies had a common area of overlap that coincided with the positions of the previously mapped escape mutations.

PubMed: 19321620
DOI: 10.1128/JVI.02532-08

実験手法

ELECTRON MICROSCOPY (11.1 Å)