3IWT

Structure of hypothetical molybdenum cofactor biosynthesis protein B from Sulfolobus tokodaii

Replaces:  2PJK

Summary for 3IWT

• Entry DOI: 10.2210/pdb3iwt/pdb
• Descriptor: 178aa long hypothetical molybdenum cofactor biosynthesis protein B, DI(HYDROXYETHYL)ETHER, MAGNESIUM ION, ... (5 entities in total)
• Functional Keywords: molybdenum cofactor, biosynthesis, structural genomics, unknown function, nppsfa, national project on protein structural and functional analyses, riken structural genomics/proteomics initiative, rsgi
• Biological source: Sulfolobus tokodaii
• Total number of polymer chains: 3
• Total formula weight: 60292.54

Authors

Antonyuk, S.V.,Ellis, M.J.,Strange, R.W.,Hasnain, S.S.,Bessho, Y.,Kuramitsu, S.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (deposition date: 2009-09-03, release date: 2009-09-29, Last modification date: 2023-11-01)

Primary citation

Antonyuk, S.V.,Strange, R.W.,Ellis, M.J.,Bessho, Y.,Kuramitsu, S.,Shinkai, A.,Yokoyama, S.,Hasnain, S.S.
Structure of hypothetical Mo-cofactor biosynthesis protein B (ST2315) from Sulfolobus tokodaii
Acta Crystallogr.,Sect.F, 65:1200-1203, 2009

PubMed Abstract: The structure of a probable Mo-cofactor biosynthesis protein B from Sulfolobus tokodaii, belonging to space group P6(4)22 with unit-cell parameters a = b = 136.68, c = 210.52 A, was solved by molecular replacement to a resolution of 1.9 A and refined to an R factor and R(free) of 16.8% and 18.5%, respectively. The asymmetric unit contains a trimer, while the biologically significant oligomer is predicted to be a hexamer by size-exclusion chromatography. The subunit structure and fold of ST2315 are similar to those of other enzymes that are known to be involved in the molybdopterin- and molybdenum cofactor-biosynthesis pathways.

PubMed: 20054111
DOI: 10.1107/S1744309109043772

Experimental method

X-RAY DIFFRACTION (1.9 Å)