3IRB

Crystal structure of protein with unknown function from DUF35 family (13815350) from SULFOLOBUS SOLFATARICUS at 1.80 A resolution

Replaces:  2GNR

Summary for 3IRB

• Entry DOI: 10.2210/pdb3irb/pdb
• Descriptor: uncharacterized protein from DUF35 family, ZINC ION, SULFATE ION, ... (5 entities in total)
• Functional Keywords: 13815350, protein with unknown function from duf35 family, structural genomics, joint center for structural genomics, jcsg, protein structure initiative, psi-2, domain of unknown function duf35, unknown function, acyl-coa binding protein
• Biological source: Sulfolobus solfataricus (Archaea)
• Total number of polymer chains: 2
• Total formula weight: 34102.69

Authors

Joint Center for Structural Genomics (JCSG) (deposition date: 2009-08-21, release date: 2009-09-01, Last modification date: 2024-11-06)

Primary citation

Krishna, S.S.,Aravind, L.,Bakolitsa, C.,Caruthers, J.,Carlton, D.,Miller, M.D.,Abdubek, P.,Astakhova, T.,Axelrod, H.L.,Chiu, H.J.,Clayton, T.,Deller, M.C.,Duan, L.,Feuerhelm, J.,Grant, J.C.,Han, G.W.,Jaroszewski, L.,Jin, K.K.,Klock, H.E.,Knuth, M.W.,Kumar, A.,Marciano, D.,McMullan, D.,Morse, A.T.,Nigoghossian, E.,Okach, L.,Reyes, R.,Rife, C.L.,van den Bedem, H.,Weekes, D.,Xu, Q.,Hodgson, K.O.,Wooley, J.,Elsliger, M.A.,Deacon, A.M.,Godzik, A.,Lesley, S.A.,Wilson, I.A.
The structure of SSO2064, the first representative of Pfam family PF01796, reveals a novel two-domain zinc-ribbon OB-fold architecture with a potential acyl-CoA-binding role.
Acta Crystallogr.,Sect.F, 66:1160-1166, 2010

PubMed Abstract: SSO2064 is the first structural representative of PF01796 (DUF35), a large prokaryotic family with a wide phylogenetic distribution. The structure reveals a novel two-domain architecture comprising an N-terminal, rubredoxin-like, zinc ribbon and a C-terminal, oligonucleotide/oligosaccharide-binding (OB) fold domain. Additional N-terminal helical segments may be involved in protein-protein interactions. Domain architectures, genomic context analysis and functional evidence from certain bacterial representatives of this family suggest that these proteins form a novel fatty-acid-binding component that is involved in the biosynthesis of lipids and polyketide antibiotics and that they possibly function as acyl-CoA-binding proteins. This structure has led to a re-evaluation of the DUF35 family, which has now been split into two entries in the latest Pfam release (v.24.0).

PubMed: 20944206
DOI: 10.1107/S1744309110002514

Experimental method

X-RAY DIFFRACTION (1.8 Å)