3IPD

Helical extension of the neuronal SNARE complex into the membrane, spacegroup I 21 21 21

「3HD9」から置き換えられました

3IPD の概要

• エントリーDOI: 10.2210/pdb3ipd/pdb
• 関連するPDBエントリー: 3HD7
• 分子名称: Vesicle-associated membrane protein 2, Syntaxin-1A, Synaptosomal-associated protein 25, ... (4 entities in total)
• 機能のキーワード: membrane protein, coiled coil, 4-helical bundle, membrane fusion, cytoplasmic vesicle, membrane, phosphoprotein, synapse, synaptosome, transmembrane, neurotransmitter transport, transport, cell membrane, cytoplasm, lipoprotein, palmitate, transport protein, acetylation, cell junction, alternative splicing, exocytosis
• 由来する生物種: Rattus norvegicus (Rat); 詳細
• 細胞内の位置: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein: P63045; Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein (By similarity): P32851; Cytoplasm, perinuclear region (By similarity): P60881 P60881
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 79787.51

構造登録者

Stein, A.,Weber, G.,Wahl, M.C.,Jahn, R. (登録日: 2009-08-17, 公開日: 2009-09-01, 最終更新日: 2023-11-01)

主引用文献

Stein, A.,Weber, G.,Wahl, M.C.,Jahn, R.
Helical extension of the neuronal SNARE complex into the membrane
Nature, 460:525-528, 2009

PubMed Abstract: Neurotransmission relies on synaptic vesicles fusing with the membrane of nerve cells to release their neurotransmitter content into the synaptic cleft, a process requiring the assembly of several members of the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) family. SNAREs represent an evolutionarily conserved protein family that mediates membrane fusion in the secretory and endocytic pathways of eukaryotic cells. On membrane contact, these proteins assemble in trans between the membranes as a bundle of four alpha-helices, with the energy released during assembly being thought to drive fusion. However, it is unclear how the energy is transferred to the membranes and whether assembly is conformationally linked to fusion. Here, we report the X-ray structure of the neuronal SNARE complex, consisting of rat syntaxin 1A, SNAP-25 and synaptobrevin 2, with the carboxy-terminal linkers and transmembrane regions at 3.4 A resolution. The structure shows that assembly proceeds beyond the already known core SNARE complex, resulting in a continuous helical bundle that is further stabilized by side-chain interactions in the linker region. Our results suggest that the final phase of SNARE assembly is directly coupled to membrane merger.

PubMed: 19571812
DOI: 10.1038/nature08156

実験手法

X-RAY DIFFRACTION (4.8 Å)