3I5Q

Nup170(aa1253-1502) at 2.2 A, S.cerevisiae

Summary for 3I5Q

• Entry DOI: 10.2210/pdb3i5q/pdb
• Related: 3I4R 3I5P
• Descriptor: Nucleoporin NUP170 (2 entities in total)
• Functional Keywords: helical stack, membrane, mrna transport, nuclear pore complex, nucleus, phosphoprotein, protein transport, translocation, transmembrane, transport
• Biological source: Saccharomyces cerevisiae (brewer's yeast,lager beer yeast,yeast)
• Cellular location: Nucleus, nuclear pore complex: P38181
• Total number of polymer chains: 2
• Total formula weight: 58558.99

Authors

Whittle, J.R.R.,Schwartz, T.U. (deposition date: 2009-07-06, release date: 2009-08-11, Last modification date: 2024-10-09)

Primary citation

Whittle, J.R.,Schwartz, T.U.
Architectural nucleoporins Nup157/170 and Nup133 are structurally related and descend from a second ancestral element.
J.Biol.Chem., 284:28442-28452, 2009

PubMed Abstract: The nuclear pore complex (NPC) constitutes one of the largest protein assemblies in the eukaryotic cell and forms the exclusive gateway to the nucleus. The stable, approximately 15-20-MDa scaffold ring of the NPC is built from two multiprotein complexes arranged around a central 8-fold axis. Here we present crystal structures of two large architectural units, yNup170(979-1502) and hNup107(658-925) x hNup133(517-1156), each a constituent of one of the two multiprotein complexes. Conservation of domain arrangement and of tertiary structure suggests that Nup157/170 and Nup133 derived from a common ancestor. Together with the previously established ancestral coatomer element (ACE1), these two elements constitute the major alpha-helical building blocks of the NPC scaffold and define its branched, lattice-like architecture, similar to vesicle coats like COPII. We hypothesize that the extant NPC evolved early during eukaryotic evolution from a rudimentary structure composed of several identical copies of a few ancestral elements, later diversified and specified by gene duplication.

PubMed: 19674973
DOI: 10.1074/jbc.M109.023580

Experimental method

X-RAY DIFFRACTION (2.204 Å)