3I3Z

Human insulin

3I3Z の概要

• エントリーDOI: 10.2210/pdb3i3z/pdb
• 関連するPDBエントリー: 3I40
• 分子名称: Insulin A chain, Insulin B chain (3 entities in total)
• 機能のキーワード: insulin, carbohydrate metabolism, cleavage on pair of basic residues, diabetes mellitus, disease mutation, disulfide bond, glucose metabolism, hormone, secreted
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Secreted: P01308 P01308
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 5787.63

構造登録者

Timofeev, V.I.,Bezuglov, V.V.,Miroshnikov, K.A.,Cuprov-Netochin, R.N.,Samigina, V.R.,Kuranova, I.P. (登録日: 2009-07-01, 公開日: 2010-01-05, 最終更新日: 2024-10-30)

主引用文献

Timofeev, V.I.,Chuprov-Netochin, R.N.,Samigina, V.R.,Bezuglov, V.V.,Miroshnikov, K.A.,Kuranova, I.P.
X-ray investigation of gene-engineered human insulin crystallized from a solution containing polysialic acid.
Acta Crystallogr.,Sect.F, 66:259-263, 2010

PubMed Abstract: Attempts to crystallize the noncovalent complex of recombinant human insulin with polysialic acid were carried out under normal and microgravity conditions. Both crystal types belonged to the same space group, I2(1)3, with unit-cell parameters a = b = c = 77.365 A, alpha = beta = gamma = 90.00 degrees. The reported space group and unit-cell parameters are almost identical to those of cubic insulin reported in the PDB. The results of X-ray studies confirmed that the crystals obtained were cubic insulin crystals and that they contained no polysialic acid or its fragments. Electron-density maps were calculated using X-ray diffraction sets from earth-grown and microgravity-grown crystals and the three-dimensional structure of the insulin molecule was determined and refined. The conformation and secondary-structural elements of the insulin molecule in different crystal forms were compared.

PubMed: 20208155
DOI: 10.1107/S1744309110000461

実験手法

X-RAY DIFFRACTION (1.6 Å)