3I08

Crystal structure of the S1-cleaved Notch1 Negative Regulatory Region (NRR)

3I08 の概要

• エントリーDOI: 10.2210/pdb3i08/pdb
• 関連するPDBエントリー: 2oo4 3eto
• 分子名称: Neurogenic locus notch homolog protein 1, CALCIUM ION, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: sea domain, lin-12 notch repeat, lnr, heterodimerization domain, hd, activator, ank repeat, calcium, cell membrane, developmental protein, differentiation, disulfide bond, egf-like domain, glycoprotein, membrane, metal-binding, notch signaling pathway, nucleus, phosphoprotein, polymorphism, receptor, transcription, transcription regulation, transmembrane, furin, t-all, leukemia, oncogene, metalloprotease, gamma-secretase, signaling protein
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cell membrane; Single-pass type I membrane protein (By similarity). Notch 1 intracellular domain: Nucleus (By similarity): P46531 P46531
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 64233.06

構造登録者

Gordon, W.R.,Blacklow, S.C. (登録日: 2009-06-24, 公開日: 2009-09-01, 最終更新日: 2024-11-06)

主引用文献

Gordon, W.R.,Vardar-Ulu, D.,L'Heureux, S.,Ashworth, T.,Malecki, M.J.,Sanchez-Irizarry, C.,McArthur, D.G.,Histen, G.,Mitchell, J.L.,Aster, J.C.,Blacklow, S.C.
Effects of S1 cleavage on the structure, surface export, and signaling activity of human Notch1 and Notch2.
Plos One, 4:e6613-e6613, 2009

PubMed Abstract: Notch receptors are normally cleaved during maturation by a furin-like protease at an extracellular site termed S1, creating a heterodimer of non-covalently associated subunits. The S1 site lies within a key negative regulatory region (NRR) of the receptor, which contains three highly conserved Lin12/Notch repeats and a heterodimerization domain (HD) that interact to prevent premature signaling in the absence of ligands. Because the role of S1 cleavage in Notch signaling remains unresolved, we investigated the effect of S1 cleavage on the structure, surface trafficking and ligand-mediated activation of human Notch1 and Notch2, as well as on ligand-independent activation of Notch1 by mutations found in human leukemia.

PubMed: 19701457
DOI: 10.1371/journal.pone.0006613

実験手法

X-RAY DIFFRACTION (3.2 Å)