3HY3

Structure of human MTHFS with 10-formyltetrahydrofolate

3HY3 の概要

• エントリーDOI: 10.2210/pdb3hy3/pdb
• 関連するPDBエントリー: 3HXT 3HY4 3HY6
• 分子名称: 5-formyltetrahydrofolate cyclo-ligase, N-({4-[{[(2R,4S,4aR,6S,8aS)-2-amino-4-hydroxydecahydropteridin-6-yl]methyl}(formyl)amino]phenyl}carbonyl)-D-glutamic acid, NICKEL (II) ION, ... (5 entities in total)
• 機能のキーワード: antifolate, cancer, 10-formyltetrahydrofolate, atp-binding, folate-binding, ligase, magnesium, nucleotide-binding
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P49914
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24105.31

構造登録者

Wu, D.,Li, Y.,Song, G.,Cheng, C.,Shaw, N.,Liu, Z.-J. (登録日: 2009-06-22, 公開日: 2009-07-14, 最終更新日: 2023-11-01)

主引用文献

Wu, D.,Li, Y.,Song, G.,Cheng, C.,Zhang, R.,Joachimiak, A.,Shaw, N.,Liu, Z.-J.
Structural basis for the inhibition of human 5,10-methenyltetrahydrofolate synthetase by N10-substituted folate analogues
Cancer Res., 69:7294-7301, 2009

PubMed Abstract: 5,10-Methenyltetrahydrofolate synthetase (MTHFS) regulates the flow of carbon through the one-carbon metabolic network, which supplies essential components for the growth and proliferation of cells. Inhibition of MTHFS in human MCF-7 breast cancer cells has been shown to arrest the growth of cells. Absence of the three-dimensional structure of human MTHFS (hMTHFS) has hampered the rational design and optimization of drug candidates. Here, we report the structures of native hMTHFS, a binary complex of hMTHFS with ADP, hMTHFS bound with the N5-iminium phosphate reaction intermediate, and an enzyme-product complex of hMTHFS. The N5-iminium phosphate captured for the first time in our crystal structure unravels a unique strategy used by hMTHFS for recognition of the substrate and provides structural basis for the regulation of enzyme activity. Binding of N10-substituted folate analogues places Y152 in the middle of the channel connecting ATP binding site with the substrate binding pocket, precluding the positioning of gamma-phosphate for a nucleophilic attack. Using the structures of hMTHFS as a guide, we have probed the role of residues surrounding the active site in catalysis by mutagenesis. The ensemble of hMTHFS structures and the mutagenesis data yield a coherent picture of the MTHFS active site, determinants of substrate specificity, and new insights into the mechanism of inhibition of hMTHFS.

PubMed: 19738041
DOI: 10.1158/0008-5472.CAN-09-1927

実験手法

X-RAY DIFFRACTION (1.8 Å)