3HWB

Cation selective pathway of OmpF porin revealed by anomalous diffraction

Summary for 3HWB

• Entry DOI: 10.2210/pdb3hwb/pdb
• Related: 3HW9
• Descriptor: Outer membrane protein F, RUBIDIUM ION, HEXAETHYLENE GLYCOL, ... (4 entities in total)
• Functional Keywords: porin, membrane protein, integral membrane protein porin, cell membrane, cell outer membrane, ion transport, membrane, phage recognition, transmembrane, transport
• Biological source: Escherichia coli
• Cellular location: Cell outer membrane; Multi-pass membrane protein: P02931
• Total number of polymer chains: 2
• Total formula weight: 80918.64

Authors

Balasundaresan, D.,Raychaudhury, S.,Blachowicz, L.,Roux, B. (deposition date: 2009-06-17, release date: 2009-12-08, Last modification date: 2023-09-06)

Primary citation

Dhakshnamoorthy, B.,Raychaudhury, S.,Blachowicz, L.,Roux, B.
Cation-selective pathway of OmpF porin revealed by anomalous X-ray diffraction.
J.Mol.Biol., 396:293-300, 2010

PubMed Abstract: The OmpF porin from the Escherichia coli outer membrane folds into a trimer of beta-barrels, each forming a wide aqueous pore allowing the passage of ions and small solutes. A long loop (L3) carrying multiple acidic residues folds into the beta-barrel pore to form a narrow "constriction zone". A strong and highly conserved charge asymmetry is observed at the constriction zone, with multiple basic residues attached to the wall of the beta-barrel (Lys16, Arg42, Arg82 and Arg132) on one side, and multiple acidic residues of L3 (Asp107, Asp113, Glu117, Asp121, Asp126, Asp127) on the other side. Several computational studies have suggested that a strong transverse electric field could exist at the constriction zone as a result of such charge asymmetry, giving rise to separate permeation pathways for cations and anions. To examine this question, OmpF was expressed, purified and crystallized in the P6(3) space group and two different data sets were obtained at 2.6 A and 3.0 A resolution with K(+) and Rb(+), respectively. The Rb(+)-soaked crystals were collected at the rubidium anomalous wavelength of 0.8149 A and cation positions were determined. A PEG molecule was observed in the pore region for both the K(+) and Rb(+)-soaked crystals, where it interacts with loop L3. The results reveal the separate pathways of anions and cations across the constriction zone of the OmpF pore.

PubMed: 19932117
DOI: 10.1016/j.jmb.2009.11.042

Experimental method

X-RAY DIFFRACTION (3 Å)