3HTU

Crystal structure of the human VPS25-VPS20 subcomplex

3HTU の概要

• エントリーDOI: 10.2210/pdb3htu/pdb
• 分子名称: Vacuolar protein-sorting-associated protein 25, Vacuolar protein-sorting-associated protein 20 (3 entities in total)
• 機能のキーワード: escrt-ii, escrt-iii, vps20, vps25, mvb, cytoplasm, nucleus, polymorphism, protein transport, transcription, transcription regulation, transport, coiled coil, endosome, lipoprotein, membrane, myristate
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm: Q9BRG1; Endomembrane system: Q96FZ7
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 54917.29

構造登録者

Im, Y.J.,Hurley, J.H. (登録日: 2009-06-12, 公開日: 2009-08-25, 最終更新日: 2023-09-06)

主引用文献

Im, Y.J.,Wollert, T.,Boura, E.,Hurley, J.H.
Structure and function of the ESCRT-II-III interface in multivesicular body biogenesis.
Dev.Cell, 17:234-243, 2009

PubMed Abstract: The ESCRT-II-ESCRT-III interaction coordinates the sorting of ubiquitinated cargo with the budding and scission of intralumenal vesicles into multivesicular bodies. The interacting regions of these complexes were mapped to the second winged helix domain of human ESCRT-II subunit VPS25 and the first helix of ESCRT-III subunit VPS20. The crystal structure of this complex was determined at 2.0 A resolution. Residues involved in structural interactions explain the specificity of ESCRT-II for Vps20, and are critical for cargo sorting in vivo. ESCRT-II directly activates ESCRT-III-driven vesicle budding and scission in vitro via these structural interactions. VPS20 and ESCRT-II bind membranes with nanomolar affinity, explaining why binding to ESCRT-II is dispensable for the recruitment of Vps20 to membranes. Docking of the ESCRT-II-VPS20(2) supercomplex reveals a convex membrane-binding surface, suggesting a hypothesis for negative membrane curvature induction in the nascent intralumenal vesicle.

PubMed: 19686684
DOI: 10.1016/j.devcel.2009.07.008

実験手法

X-RAY DIFFRACTION (2 Å)