3HP6

Crystal structure of fragment DNA polymerase I from Bacillus stearothermophilus F710Y mutant bound to G:T mismatch

3HP6 の概要

• エントリーDOI: 10.2210/pdb3hp6/pdb
• 関連するPDBエントリー: 3HPO 3HT3
• 関連するBIRD辞書のPRD_ID: PRD_900003
• 分子名称: DNA polymerase I, large fragment, 5'-D(*CP*GP*AP*TP*CP*AP*CP*GP*(DDG))-3', 5'-D(*AP*CP*GP*CP*CP*GP*TP*GP*AP*TP*CP*G)-3', ... (8 entities in total)
• 機能のキーワード: protein-dna complex, dna polymerase i, dna replication, dna-binding, dna-directed dna polymerase, hydrolase, nuclease, nucleotidyltransferase, transferase, transferase-dna complex, transferase/dna
• 由来する生物種: Geobacillus stearothermophilus; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 147172.06

構造登録者

Wu, E.Y.,Beese, L.S. (登録日: 2009-06-03, 公開日: 2010-06-23, 最終更新日: 2024-11-20)

主引用文献

Wu, E.Y.,Beese, L.S.
The structure of a high fidelity DNA polymerase bound to a mismatched nucleotide reveals an "ajar" intermediate conformation in the nucleotide selection mechanism.
J.Biol.Chem., 286:19758-19767, 2011

PubMed Abstract: To achieve accurate DNA synthesis, DNA polymerases must rapidly sample and discriminate against incorrect nucleotides. Here we report the crystal structure of a high fidelity DNA polymerase I bound to DNA primer-template caught in the act of binding a mismatched (dG:dTTP) nucleoside triphosphate. The polymerase adopts a conformation in between the previously established "open" and "closed" states. In this "ajar" conformation, the template base has moved into the insertion site but misaligns an incorrect nucleotide relative to the primer terminus. The displacement of a conserved active site tyrosine in the insertion site by the template base is accommodated by a distinctive kink in the polymerase O helix, resulting in a partially open ternary complex. We suggest that the ajar conformation allows the template to probe incoming nucleotides for complementarity before closure of the enzyme around the substrate. Based on solution fluorescence, kinetics, and crystallographic analyses of wild-type and mutant polymerases reported here, we present a three-state reaction pathway in which nucleotides either pass through this intermediate conformation to the closed conformation and catalysis or are misaligned within the intermediate, leading to destabilization of the closed conformation.

PubMed: 21454515
DOI: 10.1074/jbc.M110.191130

実験手法

X-RAY DIFFRACTION (1.81 Å)