3HOT

Crystal structure of the Mos1 mariner paired end complex with Mn

Summary for 3HOT

• Entry DOI: 10.2210/pdb3hot/pdb
• Related: 3HOS
• Descriptor: Transposable element mariner, complete cds, Mos1 NTS inverted repeat DNA, Mos1 TS inverted repeat DNA, ... (6 entities in total)
• Functional Keywords: protein-dna complex, synaptic complex, transposase, inverted repeat dna, dna binding protein-dna complex, transferase, dna binding protein/dna
• Biological source: Drosophila mauritiana (Fruit fly); More
• Total number of polymer chains: 8
• Total formula weight: 131215.02

Authors

Richardson, J.M.,Walkinshaw, M.D. (deposition date: 2009-06-03, release date: 2009-09-22, Last modification date: 2023-11-01)

Primary citation

Richardson, J.M.,Colloms, S.D.,Finnegan, D.J.,Walkinshaw, M.D.
Molecular architecture of the Mos1 paired-end complex: the structural basis of DNA transposition in a eukaryote
Cell(Cambridge,Mass.), 138:1096-1108, 2009

PubMed Abstract: A key step in cut-and-paste DNA transposition is the pairing of transposon ends before the element is excised and inserted at a new site in its host genome. Crystallographic analyses of the paired-end complex (PEC) formed from precleaved transposon ends and the transposase of the eukaryotic element Mos1 reveals two parallel ends bound to a dimeric enzyme. The complex has a trans arrangement, with each transposon end recognized by the DNA binding region of one transposase monomer and by the active site of the other monomer. Two additional DNA duplexes in the crystal indicate likely binding sites for flanking DNA. Biochemical data provide support for a model of the target capture complex and identify Arg186 to be critical for target binding. Mixing experiments indicate that a transposase dimer initiates first-strand cleavage and suggest a pathway for PEC formation.

PubMed: 19766564
DOI: 10.1016/j.cell.2009.07.012

Experimental method

X-RAY DIFFRACTION (3.25 Å)