3HM3

The Structure and conformation of Lys-63 linked tetra-ubiquitin

3HM3 の概要

• エントリーDOI: 10.2210/pdb3hm3/pdb
• 分子名称: Ubiquitin, ZINC ION (3 entities in total)
• 機能のキーワード: ubiquitin chain, lys63-linked, tetrameric ubiquitin, isopeptide bond, nucleus, phosphoprotein, signaling protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 34699.78

構造登録者

Datta, A.B.,Hura, G.L.,Wolberger, C. (登録日: 2009-05-28, 公開日: 2009-08-25, 最終更新日: 2024-11-27)

主引用文献

Datta, A.B.,Hura, G.L.,Wolberger, C.
The structure and conformation of Lys63-linked tetraubiquitin.
J.Mol.Biol., 392:1117-1124, 2009

PubMed Abstract: Ubiquitination involves the covalent attachment of the ubiquitin (Ub) C-terminus to the lysine side chain of a substrate protein by an isopeptide bond. The modification can comprise a single Ub moiety or a chain of Ub molecules joined by isopeptide bonds between the C-terminus of one Ub with one of the seven lysine residues in the next Ub. Modification of substrate proteins with Lys63-linked poly-Ub plays a key nondegradative signaling role in many biological processes, including DNA repair and nuclear factor-kappaB activation, whereas substrates modified by Lys48-linked chains are targeted to the proteasome for degradation. The distinct signaling properties of alternatively linked Ub chains presumably stem from structural differences that can be distinguished by effector proteins. We have determined the crystal structure of Lys63 tetra-Ub at a resolution of 1.96 A and performed small-angle X-ray scattering experiments and molecular dynamics simulations to probe the conformation of Lys63 tetra-Ub in solution. The chain adopts a highly extended conformation in the crystal, in contrast with the compact globular fold of Lys48 tetra-Ub. Small-angle X-ray scattering experiments show that the Lys63 tetra-Ub chain is dynamic in solution, adopting an ensemble of conformations that are more compact than the extended form in the crystal. The results of these studies provide a basis for understanding the differences in the behavior and recognition of Lys63 poly-Ub chains.

PubMed: 19664638
DOI: 10.1016/j.jmb.2009.07.090

実験手法

X-RAY DIFFRACTION (1.96 Å)