3HHN

Crystal structure of class I ligase ribozyme self-ligation product, in complex with U1A RBD

3HHN の概要

• エントリーDOI: 10.2210/pdb3hhn/pdb
• 分子名称: U1 small nuclear ribonucleoprotein A, Class I ligase ribozyme, self-ligation product, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: ligase ribozyme, ribozyme, catalytic rna, protein-rna complex, ribonucleoprotein, rna-binding, ligase-rna complex, ligase/rna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: P09012
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 111581.15

構造登録者

Shechner, D.M.,Grant, R.A.,Bagby, S.C.,Bartel, D.P. (登録日: 2009-05-15, 公開日: 2009-11-24, 最終更新日: 2024-02-21)

主引用文献

Shechner, D.M.,Grant, R.A.,Bagby, S.C.,Koldobskaya, Y.,Piccirilli, J.A.,Bartel, D.P.
Crystal structure of the catalytic core of an RNA-polymerase ribozyme.
Science, 326:1271-1275, 2009

PubMed Abstract: Primordial organisms of the putative RNA world would have required polymerase ribozymes able to replicate RNA. Known ribozymes with polymerase activity best approximating that needed for RNA replication contain at their catalytic core the class I RNA ligase, an artificial ribozyme with a catalytic rate among the fastest of known ribozymes. Here we present the 3.0 angstrom crystal structure of this ligase. The architecture resembles a tripod, its three legs converging near the ligation junction. Interacting with this tripod scaffold through a series of 10 minor-groove interactions (including two A-minor triads) is the unpaired segment that contributes to and organizes the active site. A cytosine nucleobase and two backbone phosphates abut the ligation junction; their location suggests a model for catalysis resembling that of proteinaceous polymerases.

PubMed: 19965478
DOI: 10.1126/science.1174676

実験手法

X-RAY DIFFRACTION (2.987 Å)