3HDF

Crystal structure of truncated endolysin R21 from phage 21

Summary for 3HDF

• Entry DOI: 10.2210/pdb3hdf/pdb
• Related: 3HDE
• Descriptor: Lysozyme, NITRATE ION, GLYCEROL, ... (4 entities in total)
• Functional Keywords: lysozyme-like, antimicrobial, bacteriolytic enzyme, glycosidase, hydrolase, late protein
• Biological source: Enterobacteria phage P21 (Bacteriophage 21)
• Total number of polymer chains: 2
• Total formula weight: 32648.92

Authors

Sun, Q.,Sacchettini, J.C. (deposition date: 2009-05-07, release date: 2009-11-03, Last modification date: 2024-10-16)

Primary citation

Sun, Q.,Kuty, G.F.,Arockiasamy, A.,Xu, M.,Young, R.,Sacchettini, J.C.
Regulation of a muralytic enzyme by dynamic membrane topology.
Nat.Struct.Mol.Biol., 16:1192-1194, 2009

PubMed Abstract: R(21), the lysozyme of coliphage 21, has an N-terminal signal-anchor-release (SAR) domain that directs its secretion in a membrane-tethered, inactive form and then its release and activation in the periplasm. Both genetic and crystallographic studies show that the SAR domain, once extracted from the bilayer, refolds into the body of the enzyme and effects muralytic activation by repositioning one residue of the canonical lysozyme catalytic triad.

PubMed: 19881499
DOI: 10.1038/nsmb.1681

Experimental method

X-RAY DIFFRACTION (1.7 Å)