3HDF

Crystal structure of truncated endolysin R21 from phage 21

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	120
Detector technology	CCD
Collection date	2008-06-29
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9796
Spacegroup name	P 21 21 2
Unit cell lengths	64.190, 109.673, 45.015
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.793 - 1.700
Rwork	0.200
R-free	0.23100
Structure solution method	SIRAS
RMSD bond length	0.004
RMSD bond angle	0.647
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.760
High resolution limit [Å]	1.700	3.660	1.700
Rmerge	0.038	0.020	0.372
Number of reflections	35740
<I/σ(I)>	44.163
Completeness [%]	99.9	98.9	100
Redundancy	6.1	5.8	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	100mM Bis-Tris propane, 2.5M sodium nitrate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 290K