3HDF

Crystal structure of truncated endolysin R21 from phage 21

3HDF の概要

• エントリーDOI: 10.2210/pdb3hdf/pdb
• 関連するPDBエントリー: 3HDE
• 分子名称: Lysozyme, NITRATE ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: lysozyme-like, antimicrobial, bacteriolytic enzyme, glycosidase, hydrolase, late protein
• 由来する生物種: Enterobacteria phage P21 (Bacteriophage 21)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 32648.92

構造登録者

Sun, Q.,Sacchettini, J.C. (登録日: 2009-05-07, 公開日: 2009-11-03, 最終更新日: 2024-10-16)

主引用文献

Sun, Q.,Kuty, G.F.,Arockiasamy, A.,Xu, M.,Young, R.,Sacchettini, J.C.
Regulation of a muralytic enzyme by dynamic membrane topology.
Nat.Struct.Mol.Biol., 16:1192-1194, 2009

PubMed Abstract: R(21), the lysozyme of coliphage 21, has an N-terminal signal-anchor-release (SAR) domain that directs its secretion in a membrane-tethered, inactive form and then its release and activation in the periplasm. Both genetic and crystallographic studies show that the SAR domain, once extracted from the bilayer, refolds into the body of the enzyme and effects muralytic activation by repositioning one residue of the canonical lysozyme catalytic triad.

PubMed: 19881499
DOI: 10.1038/nsmb.1681

実験手法

X-RAY DIFFRACTION (1.7 Å)