3H9X

Crystal Structure of the PSPTO_3016 protein from Pseudomonas syringae, Northeast Structural Genomics Consortium Target PsR293

Summary for 3H9X

• Entry DOI: 10.2210/pdb3h9x/pdb
• Descriptor: uncharacterized protein PSPTO_3016 (2 entities in total)
• Functional Keywords: alpha-beta protein., structural genomics, psi-2, protein structure initiative, northeast structural genomics consortium, nesg, unknown function
• Biological source: Pseudomonas syringae pv. tomato
• Total number of polymer chains: 4
• Total formula weight: 59882.30

Authors

Seetharaman, J.,Lew, S.,Forouhar, F.,Hamilton, H.,Xiao, R.,Ciccosanti, C.,Foote, E.L.,Zhao, L.,Everett, J.K.,Nair, R.,Acton, T.B.,Rost, B.,Montelione, G.T.,Hunt, J.F.,Tong, L.,Northeast Structural Genomics Consortium (NESG) (deposition date: 2009-04-30, release date: 2009-05-19, Last modification date: 2024-11-20)

Primary citation

Feldmann, E.A.,Seetharaman, J.,Ramelot, T.A.,Lew, S.,Zhao, L.,Hamilton, K.,Ciccosanti, C.,Xiao, R.,Acton, T.B.,Everett, J.K.,Tong, L.,Montelione, G.T.,Kennedy, M.A.
Solution NMR and X-ray crystal structures of Pseudomonas syringae Pspto_3016 from protein domain family PF04237 (DUF419) adopt a "double wing" DNA binding motif.
J.Struct.Funct.Genom., 13:155-162, 2012

PubMed Abstract: The protein Pspto_3016 is a 117-residue member of the protein domain family PF04237 (DUF419), which is to date a functionally uncharacterized family of proteins. In this report, we describe the structure of Pspto_3016 from Pseudomonas syringae solved by both solution NMR and X-ray crystallography at 2.5 Å resolution. In both cases, the structure of Pspto_3016 adopts a "double wing" α/β sandwich fold similar to that of protein YjbR from Escherichia coli and to the C-terminal DNA binding domain of the MotA transcription factor (MotCF) from T4 bacteriophage, along with other uncharacterized proteins. Pspto_3016 was selected by the Protein Structure Initiative of the National Institutes of Health and the Northeast Structural Genomics Consortium (NESG ID PsR293).

PubMed: 22865330
DOI: 10.1007/s10969-012-9140-8

Experimental method

X-RAY DIFFRACTION (2.51 Å)