3GYW
Crystal structure of nucleosome assembly protein from Plasmodium falciparum at 2.4 A resolution
Summary for 3GYW
| Entry DOI | 10.2210/pdb3gyw/pdb |
| Related | 3FS3 3GYV |
| Descriptor | Nucleosome assembly protein 1, putative (2 entities in total) |
| Functional Keywords | sir, siras, nucleosome assembly protein, histone recognition, chaperone |
| Biological source | Plasmodium falciparum |
| Total number of polymer chains | 1 |
| Total formula weight | 29667.74 |
| Authors | Yogavel, M.,Gill, J.,Sharma, A. (deposition date: 2009-04-06, release date: 2009-05-12, Last modification date: 2024-03-20) |
| Primary citation | Yogavel, M.,Gill, J.,Sharma, A. Iodide-SAD, SIR and SIRAS phasing for structure solution of a nucleosome assembly protein. Acta Crystallogr.,Sect.D, 65:618-622, 2009 Cited by PubMed Abstract: The crystal structure of Plasmodium falciparum nucleosome assembly protein (PfNapL) was determined by iodide-SAD/SIRAS phasing methods using iodide-SAD data to 3.0 A resolution and native data to 2.4 A resolution. Halide-derivatized PfNapL crystals were obtained using the quick cryo-soaking method in which the native crystals were soaked in a cryosolution consisting of 500 mM NaI for a short period of 30-60 s and data were collected at an in-house X-ray source using Cu Kalpha radiation. Despite a low anomalous signal-to-noise ratio of <1.2 in the >3.5 A resolution bin, the data were sufficient to determine the structure by SAD/SIR/SIRAS methods using the soaked iodides. Previously, structure solution had failed with both molecular-replacement and selenomethionine-derivatization techniques owing to reasons that are detailed in this work. The phasing at low resolution with three iodides per monomer with high temperature factors was successful using any of the SAD, SIR or SIRAS methods. PubMed: 19465776DOI: 10.1107/S0907444909013171 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.4 Å) |
Structure validation
Download full validation report
