3GUO

T4 lysozyme M102E/L99A mutant with buried charge in apolar cavity--phenol binding

Summary for 3GUO

• Entry DOI: 10.2210/pdb3guo/pdb
• Related: 3GUI 3GUJ 3GUK 3GUL 3GUM 3GUN 3GUP
• Descriptor: Lysozyme, PHENOL, CHLORIDE ION, ... (5 entities in total)
• Functional Keywords: t4 lysozyme, apolar cavity, buried charge, ligand binding, antimicrobial, bacteriolytic enzyme, glycosidase, hydrolase
• Biological source: Enterobacteria phage T4
• Cellular location: Host cytoplasm : P00720
• Total number of polymer chains: 2
• Total formula weight: 37753.79

Authors

Liu, L.,Matthews, B.W. (deposition date: 2009-03-30, release date: 2009-08-25, Last modification date: 2023-09-06)

• Primary citation: Liu, L.,Baase, W.A.,Michael, M.M.,Matthews, B.W.; Use of stabilizing mutations to engineer a charged group within a ligand-binding hydrophobic cavity in T4 lysozyme.; Biochemistry, 48:8842-8851, 2009; PubMed: 19663503; DOI: 10.1021/bi900685j

Experimental method

X-RAY DIFFRACTION (2.16 Å)