3G5O

The crystal structure of the toxin-antitoxin complex RelBE2 (Rv2865-2866) from Mycobacterium tuberculosis

3G5O の概要

• エントリーDOI: 10.2210/pdb3g5o/pdb
• 分子名称: Uncharacterized protein Rv2865, Uncharacterized protein Rv2866, THIOCYANATE ION, ... (8 entities in total)
• 機能のキーワード: heterotetramer, 1:1 ratio, structural genomics, psi-2, protein structure initiative, tb structural genomics consortium, tbsgc, integrated center for structure and function innovation, isfi, toxin-antitoxin complex, toxin/antitoxin
• 由来する生物種: Mycobacterium tuberculosis; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 48248.83

構造登録者

Miallau, L.,Cascio, D.,Eisenberg, D.,TB Structural Genomics Consortium (TBSGC),Integrated Center for Structure and Function Innovation (ISFI) (登録日: 2009-02-05, 公開日: 2009-04-14, 最終更新日: 2024-05-22)

主引用文献

Miallau, L.,Jain, P.,Arbing, M.A.,Cascio, D.,Phan, T.,Ahn, C.J.,Chan, S.,Chernishof, I.,Maxson, M.,Chiang, J.,Jacobs Jr., W.R.,Eisenberg, D.S.
Comparative proteomics identifies the cell-associated lethality of M. tuberculosis RelBE-like toxin-antitoxin complexes.
Structure, 21:627-637, 2013

PubMed Abstract: The Mycobacterium tuberculosis (Mtb) genome encodes approximately 90 toxin-antitoxin protein complexes, including three RelBE family members, which are believed to play a major role in bacterial fitness and pathogenicity. We have determined the crystal structures of Mtb RelBE-2 and RelBE-3, and the structures reveal homologous heterotetramers. Our structures suggest RelE-2, and by extension the closely related RelE-1, use a different catalytic mechanism than RelE-3, because our analysis of the RelE-2 structure predicts additional amino acid residues that are likely to be functionally significant and are missing from analogous positions in the RelE-3 structure. Toxicity assays corroborate our structural findings; overexpression of RelE-3, whose active site is more similar to Escherichia coli YoeB, has limited consequences on bacterial growth, whereas RelE-1 and RelE-2 overexpression results in acute toxicity. Moreover, RelE-2 overexpression results in an elongated cell phenotype in Mycobacterium smegmatis and protects M. tuberculosis against antibiotics, suggesting a different functional role for RelE-2.

PubMed: 23523424
DOI: 10.1016/j.str.2013.02.008

実験手法

X-RAY DIFFRACTION (2 Å)