3FKU

Crystal structure of influenza hemagglutinin (H5) in complex with a broadly neutralizing antibody F10

3FKU の概要

• エントリーDOI: 10.2210/pdb3fku/pdb
• 関連するPDBエントリー: 2FK0 2GHW 2IBX
• 分子名称: Hemagglutinin, Hemagglutinin HA2 chain, Neutralizing antibody F10, ... (5 entities in total)
• 機能のキーワード: influenza, hemagglutinin, neutralizing antibody, scfv, h5, f10, cell membrane, envelope protein, fusion protein, membrane, transmembrane, virion, cleavage on pair of basic residues, glycoprotein, lipoprotein, palmitate, viral protein, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Influenza A virus (A/Viet Nam/1203/2004(H5N1)); 詳細
• 細胞内の位置: Virion membrane; Single-pass type I membrane protein (Potential): A8UDR4
• タンパク質・核酸の鎖数: 18
• 化学式量合計: 538427.88

構造登録者

Hwang, W.C.,Santelli, E.,Stec, B.,Wei, G.,Cadwell, G.,Bankston, L.A.,Sui, J.,Perez, S.,Aird, D.,Chen, L.M.,Ali, M.,Murakami, A.,Yammanuru, A.,Han, T.,Cox, N.,Donis, R.O.,Liddington, R.C.,Marasco, W.A. (登録日: 2008-12-17, 公開日: 2009-02-24, 最終更新日: 2024-12-25)

主引用文献

Sui, J.,Hwang, W.C.,Perez, S.,Wei, G.,Aird, D.,Chen, L.M.,Santelli, E.,Stec, B.,Cadwell, G.,Ali, M.,Wan, H.,Murakami, A.,Yammanuru, A.,Han, T.,Cox, N.J.,Bankston, L.A.,Donis, R.O.,Liddington, R.C.,Marasco, W.A.
Structural and functional bases for broad-spectrum neutralization of avian and human influenza A viruses.
Nat.Struct.Mol.Biol., 16:265-273, 2009

PubMed Abstract: Influenza virus remains a serious health threat, owing to its ability to evade immune surveillance through rapid genetic drift and reassortment. Here we used a human non-immune antibody phage-display library and the H5 hemagglutinin ectodomain to select ten neutralizing antibodies (nAbs) that were effective against all group 1 influenza viruses tested, including H5N1 'bird flu' and the H1N1 'Spanish flu'. The crystal structure of one such nAb bound to H5 shows that it blocks infection by inserting its heavy chain into a conserved pocket in the stem region, thus preventing membrane fusion. Nine of the nAbs employ the germline gene VH1-69, and all seem to use the same neutralizing mechanism. Our data further suggest that this region is recalcitrant to neutralization escape and that nAb-based immunotherapy is a promising strategy for broad-spectrum protection against seasonal and pandemic influenza viruses.

PubMed: 19234466
DOI: 10.1038/nsmb.1566

実験手法

X-RAY DIFFRACTION (3.2 Å)