3FFV

Crystal Structure Analysis of Syd

3FFV の概要

• エントリーDOI: 10.2210/pdb3ffv/pdb
• 分子名称: Protein syd (2 entities in total)
• 機能のキーワード: membrane, translocon, secyeg, syd, nanodisc, cell inner membrane, cell membrane, protein binding
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side: P0A8U0
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 41458.84

構造登録者

Maurus, R.,Brayer, G.D. (登録日: 2008-12-04, 公開日: 2009-01-27, 最終更新日: 2011-07-13)

主引用文献

Dalal, K.,Nguyen, N.,Alami, M.,Tan, J.,Moraes, T.F.,Lee, W.C.,Maurus, R.,Sligar, S.S.,Brayer, G.D.,Duong, F.
Structure, Binding, and Activity of Syd, a SecY-interacting Protein
J.Biol.Chem., 284:7897-7902, 2009

PubMed Abstract: The Syd protein has been implicated in the Sec-dependent transport of polypeptides across the bacterial inner membrane. Using Nanodiscs, we here provide direct evidence that Syd binds the SecY complex, and we demonstrate that interaction involves the two electropositive and cytosolic loops of the SecY subunit. We solve the crystal structure of Syd and together with cysteine cross-link analysis, we show that a conserved concave and electronegative groove constitutes the SecY-binding site. At the membrane, Syd decreases the activity of the translocon containing loosely associated SecY-SecE subunits, whereas in detergent solution Syd disrupts the SecYEG heterotrimeric associations. These results support the role of Syd in proofreading the SecY complex biogenesis and point to the electrostatic nature of the Sec channel interaction with its cytosolic partners.

PubMed: 19139097
DOI: 10.1074/jbc.M808305200

実験手法

X-RAY DIFFRACTION (2 Å)