3FDY

Pyranose 2-oxidase thermostable triple mutant, T169G/E542K/V546C

3FDY の概要

• エントリーDOI: 10.2210/pdb3fdy/pdb
• 関連するPDBエントリー: 2IGK 2IGM 2IGN 2IGO 3BG6 3BLY
• 分子名称: Pyranose oxidase (Pyranose 2-oxidase), FLAVIN-ADENINE DINUCLEOTIDE, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, ... (4 entities in total)
• 機能のキーワード: pyranose oxidase, thermostable, mutant, gmc oxidoreductase, 8-alpha-(n3) histidyl flavinylation, oxidoreductase
• 由来する生物種: Trametes multicolor (White-rot fungus)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 70354.59

構造登録者

Tan, T.C.,Divne, C. (登録日: 2008-11-26, 公開日: 2009-03-31, 最終更新日: 2024-10-30)

主引用文献

Spadiut, O.,Radakovits, K.,Pisanelli, I.,Salaheddin, C.,Yamabhai, M.,Tan, T.-C.,Divne, C.,Haltrich, D.
A thermostable triple mutant of pyranose 2-oxidase from Trametes multicolor with improved properties for biotechnological applications
Biotechnol J, 4:525-534, 2009

PubMed Abstract: In order to increase the thermal stability and the catalytic properties of pyranose oxidase (P2Ox) from Trametes multicolor toward its poor substrate D-galactose and the alternative electron acceptor 1,4-benzoquinone (1,4-BQ), we designed the triple-mutant T169G/E542K/V546C. Whereas the wild-type enzyme clearly favors D-glucose as its substrate over D-galactose [substrate selectivity (k(cat)/K(M))(Glc)/(k(cat)/K(M))(Gal) = 172], the variant oxidizes both sugars equally well [(k(cat)/K(M))(Glc)/(k(cat)/K(M))(Gal) = 0.69], which is of interest for food biotechnology. Furthermore, the variant showed lower K(M) values and approximately ten-fold higher k(cat) values for 1,4-BQ when D-galactose was used as the saturating sugar substrate, which makes this enzyme particularly attractive for use in biofuel cells and enzyme-based biosensors. In addition to the altered substrate specificity and reactivity, this mutant also shows significantly improved thermal stability. The half life time at 60 degrees C was approximately 10 h, compared to 7.6 min for the wild-type enzyme. We performed successfully small-scale bioreactor pilot conversion experiments of D-glucose/D-galactose mixtures at both 30 and 50 degrees C, showing the usefulness of this P2Ox variant in biocatalysis as well as the enhanced thermal stability of the enzyme. Moreover, we determined the crystal structure of the mutant in its unligated form at 1.55 A resolution. Modeling D-galactose in position for oxidation at C2 into the mutant active site shows that substituting Thr for Gly at position 169 favorably accommodates the axial C4 hydroxyl group that would otherwise clash with Thr169 in the wild-type.

PubMed: 19291706
DOI: 10.1002/biot.200800260

実験手法

X-RAY DIFFRACTION (1.55 Å)