3FCT

MATURE METAL CHELATASE CATALYTIC ANTIBODY WITH HAPTEN

Summary for 3FCT

• Entry DOI: 10.2210/pdb3fct/pdb
• Descriptor: PROTEIN (METAL CHELATASE CATALYTIC ANTIBODY), CADMIUM ION, CALCIUM ION, ... (8 entities in total)
• Functional Keywords: metal chelatase, catalytic antibody, fab fragment, immune system
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 4
• Total formula weight: 94557.33

Authors

Romesberg, F.E.,Santarsiero, B.D.,Barnes, D.,Yin, J.,Spiller, B.,Schultz, P.G.,Stevens, R.C. (deposition date: 1999-06-13, release date: 1999-06-23, Last modification date: 2024-11-20)

Primary citation

Romesberg, F.E.,Santarsiero, B.D.,Spiller, B.,Yin, J.,Barnes, D.,Schultz, P.G.,Stevens, R.C.
Structural and kinetic evidence for strain in biological catalysis.
Biochemistry, 37:14404-14409, 1998

PubMed Abstract: A classic hypothesis for enzyme catalysis is the induction of strain in the substrate. This notion was first expressed by Haldane with the lock and key analogy-"the key does not fit the lock perfectly but exercises a certain strain on it" (1). This mechanism has often been invoked to explain the catalytic efficiency of enzymes but has been difficult to establish conclusively (2-7). Here we describe X-ray crystallographic and mutational studies of an antibody metal chelatase which strongly support the notion that this antibody catalyzes metal ion insertion into the porphyrin ring by inducing strain. Analysis of the germline precursor suggests that this strain mechanism arose during the process of affinity maturation in response to a conformationally distorted N-alkylmesoporphyrin.

PubMed: 9772166
DOI: 10.1021/bi981578c

Experimental method

X-RAY DIFFRACTION (2.4 Å)