3FC0

1.8 A crystal structure of murine GITR ligand dimer expressed in Drosophila melanogaster S2 cells

3FC0 の概要

• エントリーDOI: 10.2210/pdb3fc0/pdb
• 関連するPDBエントリー: 2QDN
• 分子名称: GITR ligand, ACETATE ION (3 entities in total)
• 機能のキーワード: gitrl; glucocorticoid-induced tnf receptor ligand, receptor, protein binding
• 由来する生物種: Mus musculus (mouse)
• 細胞内の位置: Cell membrane ; Single-pass type II membrane protein : Q7TS55
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 31863.64

構造登録者

Chattopadhyay, K.,Ramagopal, U.A.,Nathenson, S.G.,Almo, S.C. (登録日: 2008-11-20, 公開日: 2008-12-30, 最終更新日: 2024-10-16)

主引用文献

Chattopadhyay, K.,Ramagopal, U.A.,Nathenson, S.G.,Almo, S.C.
1.8 A structure of murine GITR ligand dimer expressed in Drosophila melanogaster S2 cells.
Acta Crystallogr.,Sect.D, 65:434-439, 2009

PubMed Abstract: Glucocorticoid-induced TNF receptor ligand (GITRL), a prominent member of the TNF superfamily, activates its receptor on both effector and regulatory T cells to generate critical costimulatory signals that have been implicated in a wide range of T-cell immune functions. The crystal structures of murine and human orthologs of GITRL recombinantly expressed in Escherichia coli have previously been determined. In contrast to all classical TNF structures, including the human GITRL structure, murine GITRL demonstrated a unique ;strand-exchanged' dimeric organization. Such a novel assembly behavior indicated a dramatic impact on receptor activation as well as on the signaling mechanism associated with the murine GITRL costimulatory system. In this present work, the 1.8 A resolution crystal structure of murine GITRL expressed in Drosophila melanogaster S2 cells is reported. The eukaryotic protein-expression system allows transport of the recombinant protein into the extracellular culture medium, thus maximizing the possibility of obtaining correctly folded material devoid of any folding/assembly artifacts that are often suspected with E. coli-expressed proteins. The S2 cell-expressed murine GITRL adopts an identical ;strand-exchanged' dimeric structure to that observed for the E. coli-expressed protein, thus conclusively demonstrating the novel quaternary structure assembly behavior of murine GITRL.

PubMed: 19390148
DOI: 10.1107/S0907444909005721

実験手法

X-RAY DIFFRACTION (1.76 Å)