3FBD

Crystal structure of the nuclease domain of COLE7(D493Q mutant) in complex with an 18-BP duplex DNA

3FBD の概要

• エントリーDOI: 10.2210/pdb3fbd/pdb
• 関連するPDBエントリー: 1PT3 1ZNS 2IVH
• 分子名称: Colicin-E7, 5'-D(*DGP*DGP*DAP*DAP*DTP*DTP*DCP*DGP*DAP*DTP*DCP*DGP*DAP*DAP*DTP*DTP*DCP*DC)-3' (3 entities in total)
• 機能のキーワード: computational redesign, protein engineering, protein-nucleic acid interactions, dnase, dna hydrolysis., antibiotic, antimicrobial, bacteriocin, endonuclease, hydrolase, metal-binding, nuclease, plasmid, zinc, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 52386.81

構造登録者

Wang, Y.T.,Doudeva, L.G.,Yuan, H.S. (登録日: 2008-11-19, 公開日: 2009-11-03, 最終更新日: 2023-11-01)

• 主引用文献: Wang, Y.T.,Wright, J.D.,Doudeva, L.G.,Jhang, H.C.,Lim, C.,Yuan, H.S.; Redesign of high-affinity nonspecific nucleases with altered sequence preference; To be Published,

実験手法

X-RAY DIFFRACTION (2.9 Å)